Cell Death Regulation by Smed-foxO in the Planarian Schmidtea Mediterranea

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Nov 20

PMID 39565589

Authors

Maria Rossello

Teresa Adell

Eudald Pascual-Carreras

Affiliations

Soon will be listed here.

Abstract

The planarian Schmidtea mediterranea shows nutrient-dependent whole-body plasticity. Starvation leads to body size reduction, while feeding triggers growth. The balance of cell proliferation and cell death controls cell number, driving organismal body size. Here, we uncovered the role of FoxO in controlling cell death through TUNEL and caspase-3 assays and bak qPCR detection in foxO RNAi planarians.

References

Pellettieri J, Fitzgerald P, Watanabe S, Mancuso J, Green D, Sanchez Alvarado A . Cell death and tissue remodeling in planarian regeneration. Dev Biol. 2009; 338(1):76-85. PMC: 2835816. DOI: 10.1016/j.ydbio.2009.09.015. View

Baguna J . Dramatic mitotic response in planarians after feeding, and a hypothesis for the control mechanism. J Exp Zool. 1974; 190(1):117-22. DOI: 10.1002/jez.1401900111. View

Newmark P, Sanchez Alvarado A . Bromodeoxyuridine specifically labels the regenerative stem cells of planarians. Dev Biol. 2001; 220(2):142-53. DOI: 10.1006/dbio.2000.9645. View

Tu K, Pearson B, Sanchez Alvarado A . TORC1 is required to balance cell proliferation and cell death in planarians. Dev Biol. 2012; 365(2):458-69. PMC: 3367000. DOI: 10.1016/j.ydbio.2012.03.010. View

Peiris T, Ramirez D, Barghouth P, Oviedo N . The Akt signaling pathway is required for tissue maintenance and regeneration in planarians. BMC Dev Biol. 2016; 16:7. PMC: 4827215. DOI: 10.1186/s12861-016-0107-z. View

Almuedo-Castillo M, Crespo-Yanez X, Crespo X, Seebeck F, Bartscherer K, Salo E . JNK controls the onset of mitosis in planarian stem cells and triggers apoptotic cell death required for regeneration and remodeling. PLoS Genet. 2014; 10(6):e1004400. PMC: 4055413. DOI: 10.1371/journal.pgen.1004400. View

Du S, Zheng H . Role of FoxO transcription factors in aging and age-related metabolic and neurodegenerative diseases. Cell Biosci. 2021; 11(1):188. PMC: 8561869. DOI: 10.1186/s13578-021-00700-7. View

Pascual-Carreras E, Herrera-Ubeda C, Rossello M, Coronel-Cordoba P, Garcia-Fernandez J, Salo E . Analysis of Fox genes in Schmidtea mediterranea reveals new families and a conserved role of Smed-foxO in controlling cell death. Sci Rep. 2021; 11(1):2947. PMC: 7859237. DOI: 10.1038/s41598-020-80627-0. View

Sanchez Alvarado A, Newmark P . Double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc Natl Acad Sci U S A. 1999; 96(9):5049-54. PMC: 21814. DOI: 10.1073/pnas.96.9.5049. View

10.

Levine B, Sinha S, Kroemer G . Bcl-2 family members: dual regulators of apoptosis and autophagy. Autophagy. 2008; 4(5):600-6. PMC: 2749577. DOI: 10.4161/auto.6260. View

11.

Castillo-Lara S, Pascual-Carreras E, Abril J . PlanExp: intuitive integration of complex RNA-seq datasets with planarian omics resources. Bioinformatics. 2019; 36(6):1889-1895. DOI: 10.1093/bioinformatics/btz802. View

12.

Pascual-Carreras E, Marin-Barba M, Castillo-Lara S, Coronel-Cordoba P, Magri M, Wheeler G . Wnt/β-catenin signalling is required for pole-specific chromatin remodeling during planarian regeneration. Nat Commun. 2023; 14(1):298. PMC: 9849279. DOI: 10.1038/s41467-023-35937-y. View