Tracking Insulin- and Glucagon-Expressing Cells In Vitro and In Vivo Using a Double-Reporter Human Embryonic Stem Cell Line

Overview

Journal Diabetes

Specialty Endocrinology

Date 2024 Nov 19

PMID 39561351

Authors

Samantha Mar

Ekaterina Filatov

Shugo Sasaki

Majid Mojibian

Dahai Zhang

Angela Yang

Cuilan Nian

Francis C Lynn

Affiliations

Soon will be listed here.

Abstract

Differentiation protocols used to generate stem cell-derived islet cells yield heterogenous cell populations. We generated a human embryonic stem cell line that reports insulin- and glucagon-expressing cells in vitro and in vivo without altering their differentiation or function. We showed some insulin- and glucagon-expressing bihormonal cells are cell-autonomously fated to become α-like cells. This reporter cell line can be used to further study and improve stem cell-derived islet differentiation and transplantation.

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