The Atypical Soluble Guanylyl Cyclase Subunit Gyc89Db Does Not Control Neuroepithelial Proliferation in Larval Brain

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2024 Nov 18

PMID 39554806

Authors

Mariel Rosas

Rafael Cantera

Daniel Prieto

Affiliations

Soon will be listed here.

Abstract

We investigated the role of oxygen-sensing atypical guanylyl cyclase subunit Gyc89Db in the developing brain. Despite its expression in the hypoxic neuroepithelium of the larval optic lobe of , loss-of-function mutants and ectopic expression did not alter neuroepithelial cell number or proliferation. Notably, while ectopic expression of increases optic lobe volume and neuroblast numbers, our negative results suggest that these effects manifest earlier in development without persistent alteration of the neuroepithelium, through mechanisms that might be independent of neuroepithelial proliferation.

References

Yasugi T, Umetsu D, Murakami S, Sato M, Tabata T . Drosophila optic lobe neuroblasts triggered by a wave of proneural gene expression that is negatively regulated by JAK/STAT. Development. 2008; 135(8):1471-80. DOI: 10.1242/dev.019117. View

Cardona A, Saalfeld S, Schindelin J, Arganda-Carreras I, Preibisch S, Longair M . TrakEM2 software for neural circuit reconstruction. PLoS One. 2012; 7(6):e38011. PMC: 3378562. DOI: 10.1371/journal.pone.0038011. View

Izquierdo M . Ubiquitin genes and ubiquitin protein location in polytene chromosomes of Drosophila. Chromosoma. 1994; 103(3):193-7. DOI: 10.1007/BF00368012. View

Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T . Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012; 9(7):676-82. PMC: 3855844. DOI: 10.1038/nmeth.2019. View

Morton D, Langlais K, Stewart J, Vermehren A . Comparison of the properties of the five soluble guanylyl cyclase subunits in Drosophila melanogaster. J Insect Sci. 2005; 5:12. PMC: 1307573. DOI: 10.1093/jis/5.1.12. View

Ferreiro M, Perez C, Marchesano M, Ruiz S, Caputi A, Aguilera P . Mutant Undergo Retinal Degeneration. Front Neurosci. 2018; 11:732. PMC: 5758589. DOI: 10.3389/fnins.2017.00732. View

Baccino-Calace M, Prieto D, Cantera R, Egger B . Compartment and cell-type specific hypoxia responses in the developing brain. Biol Open. 2020; 9(8). PMC: 7449796. DOI: 10.1242/bio.053629. View

Prieto D, Egger B, Cantera R . Atypical soluble guanylyl cyclases control brain size in . MicroPubl Biol. 2024; 2024. PMC: 11344882. DOI: 10.17912/micropub.biology.001252. View

Vermehren-Schmaedick A, Ainsley J, Johnson W, Davies S, Morton D . Behavioral responses to hypoxia in Drosophila larvae are mediated by atypical soluble guanylyl cyclases. Genetics. 2010; 186(1):183-96. PMC: 2940286. DOI: 10.1534/genetics.110.118166. View

10.

Morton D, Vermehren A . Soluble Guanylyl Cyclases in Invertebrates: Targets for NO and O(2). Adv Exp Biol. 2009; 1:65-82. PMC: 2613308. DOI: 10.1016/S1872-2423(07)01003-4. View

11.

Morton D, Stewart J, Langlais K, Clemens-Grisham R, Vermehren A . Synaptic transmission in neurons that express the Drosophila atypical soluble guanylyl cyclases, Gyc-89Da and Gyc-89Db, is necessary for the successful completion of larval and adult ecdysis. J Exp Biol. 2008; 211(Pt 10):1645-56. PMC: 2424211. DOI: 10.1242/jeb.014472. View

12.

Prieto D, Aparicio G, Sotelo-Silveira J . Cell migration analysis: A low-cost laboratory experiment for cell and developmental biology courses using keratocytes from fish scales. Biochem Mol Biol Educ. 2017; 45(6):475-482. DOI: 10.1002/bmb.21071. View

13.

Morton D . Behavioral responses to hypoxia and hyperoxia in Drosophila larvae: molecular and neuronal sensors. Fly (Austin). 2010; 5(2):119-25. PMC: 3127060. DOI: 10.4161/fly.5.2.14284. View

14.

Egger B, Boone J, Stevens N, Brand A, Doe C . Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe. Neural Dev. 2007; 2:1. PMC: 1779784. DOI: 10.1186/1749-8104-2-1. View