Virus Susceptibility of a New Cell Line Derived from the Muscle of Koi (Cyprinus Carpio Koi)

Overview

Journal Arch Virol

Specialty Microbiology

Date 2024 Nov 13

PMID 39538029

Authors

Hongli Jing

Lin Mei

Jizhou Lv

Min Zhang

Na Wang

Lipu Xu

Shaoqiang Wu

Affiliations

Soon will be listed here.

Abstract

In this study, a continuous cell line (KM cells) derived from koi (Cyprinus carpio koi) muscle was established and characterized. The KM cells were subcultured for more than 70 passages and showed high viability after long-term cryopreservation. The KM cell line was optimally cultured in medium 199 containing 10% foetal bovine serum at 25°C. A chromosome analysis indicated that the cell line remained diploid, with a mean chromosome count of 100. DNA sequencing and comparative analysis of the 16S rRNA and cytochrome oxidase I gene sequences showed that the KM cell line originated from koi. In transfection experiments using the plasmid pEGFP, KM cells demonstrated a high level of transfection efficiency, suggesting their potential for use in foreign gene expression studies. Inoculation with spring viraemia of carp virus (SVCV) resulted in a substantial cytopathic effect, and the level of production of SVCV in KM cells was higher than that in the epithelioma papulosum cyprinid (EPC) cell line that is normally used to produce the virus. However, no cytopathic effect was observed when these cells were inoculated with koi herpesvirus, carp oedema virus, or grass carp reovirus. These observations suggest that the newly established KM cell line will be a valuable tool for investigating the pathogenesis of infection with spring viraemia of carp virus.

Citing Articles

Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi).

Jing H, Mei L, Lv J, Zhang M, Wang N, Xu L Arch Virol. 2024; 169(12):243.

PMID: 39538029 DOI: 10.1007/s00705-024-06168-3.

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