Exploring the Siderophore Portfolio for Mass Spectrometry-Based Diagnosis of Scedosporiosis and Lomentosporiosis

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Nov 11

PMID 39524635

Authors

Jiri Houst

Andrea Palyzova

Tomas Pluhacek

Jiri Novak

Helena Maresova

Petr Hubacek

Radim Dobias

David A Stevens

Helene Guegan

Jean-Pierre Gangneux

Vladimir Havlicek

Affiliations

Soon will be listed here.

Abstract

and secrete siderophores (iron scavengers) during hyphal proliferation. Siderophores are virulence factors and potential clinical biomarkers of invasive scedosporiosis and lomentosporiosis. Both strains secreted a uniform spectrum of siderophores, including coprogen B (CopB), -methyl-coprogen B, dimethyl-coprogen, and ferricrocin, with -methyl-coprogen B being the fastest secreted and most abundant coprogen. Under iron and zinc restriction, reflecting a nutrient-limited host environment, secreted 45 times more CopB than did , presumably contributing to its higher virulence. This robust mobilization of CopB was further enhanced by zinc surplus. Additionally, two novel cyclic peptides, Scedocyclin A and B, were characterized in using the sequencing tool CycloBranch. Utilizing matrix-assisted laser desorption/ionization, the portfolio of coprogens detected had limits of detection and quantitation of 4.9 and 14.6 fmol/spot in complex matrices, respectively, making them strong candidates for the next-generation, routine diagnosis of invasive scedosporiosis and lomentosporiosis through the Biotyper siderotyping.

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