Integrative Metabolomic and Transcriptomic Analysis Provides Novel Insights into the Effects of SO on the Postharvest Quality of 'Munage' Table Grapes

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Nov 9

PMID 39517277

Authors

Zhenliang Mou

Yuyao Yuan

Wei Wei

Yating Zhao

Bin Wu

Jianye Chen

Affiliations

Soon will be listed here.

Abstract

Postharvest grapes exhibit a limited shelf life due to susceptibility to rot and deterioration, significantly reducing their nutritional and economic value. Sulfur dioxide (SO) is a widely recognized preservative for extending grape storage life. This study performed a detailed analysis of 'Munage' table grapes treated with SO fumigation, employing transcriptomic and metabolomic approaches. Results indicate that SO fumigation significantly extends the shelf life of grapes, as demonstrated by improved visual quality, reduced decay rates, and increased fruit firmness. We identified 309 differentially accumulated metabolites (DAMs) and 1906 differentially expressed genes (DEGs), including 135 transcription factors (TFs). Both DEGs and DAMs showed significant enrichment of flavonoid-related metabolism compared with the control, and the relative content of four flavonoid metabolites (Wogonin-7-O-glucuronide, Acacetin-7-O-glucuronide, Apigenin-7-O-glucuronide, and Baicalein 7-O-glucuronide) were significantly increased in grapes upon SO treatment, suggesting that SO treatment had a substantial regulatory effect on grape flavonoid metabolism. Importantly, we constructed complex regulatory networks by screening key enzyme genes (e.g., , , , , and ) related to the metabolism of target flavonoid, as well as potential regulatory transcription factors (TFs). Overall, our findings offer new insights into the regulatory mechanisms by which SO maintains the postharvest quality of table grapes.

References

Ju Y, Wang W, Yue X, Xue W, Zhang Y, Fang Y . Integrated metabolomic and transcriptomic analysis reveals the mechanism underlying the accumulation of anthocyanins and other flavonoids in the flesh and skin of teinturier grapes. Plant Physiol Biochem. 2023; 197:107667. DOI: 10.1016/j.plaphy.2023.107667. View

Donnez D, Jeandet P, Clement C, Courot E . Bioproduction of resveratrol and stilbene derivatives by plant cells and microorganisms. Trends Biotechnol. 2009; 27(12):706-13. DOI: 10.1016/j.tibtech.2009.09.005. View

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

Shojaeifard Z, Hemmateenejad B, Jassbi A . Chemometrics-based LC-UV-ESIMS analyses of 50 Salvia species for detecting their antioxidant constituents. J Pharm Biomed Anal. 2020; 193:113745. DOI: 10.1016/j.jpba.2020.113745. View

Wan H, Liu Y, Wang T, Jiang P, Wen W, Nie J . Combined transcriptomic and metabolomic analyses identifies CsERF003, a citrus ERF transcription factor, as flavonoid activator. Plant Sci. 2023; 334:111762. DOI: 10.1016/j.plantsci.2023.111762. View

Song M, Wang L, Zhang Y, Wang Q, Han X, Yang Q . Temporospatial pattern of flavonoid metabolites and potential regulatory pathway of PbMYB211-coordinated kaempferol-3-O-rhamnoside biosynthesis in Phoebe bournei. Plant Physiol Biochem. 2023; 202:107913. DOI: 10.1016/j.plaphy.2023.107913. View

Wang Y, Zhang Y, Guo Y, Ji N, Chen Y, Sun Y . Integrated transcriptomic and metabolomic analysis reveals the effects and potential mechanism of hydrogen peroxide on pigment metabolism in postharvest broccoli. J Food Sci. 2024; 89(10):6189-6202. DOI: 10.1111/1750-3841.17308. View

Ma W, Xu L, Gao S, Lyu X, Cao X, Yao Y . Melatonin alters the secondary metabolite profile of grape berry skin by promoting VvMYB14-mediated ethylene biosynthesis. Hortic Res. 2021; 8(1):43. PMC: 7917092. DOI: 10.1038/s41438-021-00478-2. View

Zhang K, Li W, Ju Y, Wang X, Sun X, Fang Y . Transcriptomic and Metabolomic Basis of Short- and Long-Term Post-Harvest UV-C Application in Regulating Grape Berry Quality Development. Foods. 2021; 10(3). PMC: 8001394. DOI: 10.3390/foods10030625. View

10.

Saito K, Yonekura-Sakakibara K, Nakabayashi R, Higashi Y, Yamazaki M, Tohge T . The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity. Plant Physiol Biochem. 2013; 72:21-34. DOI: 10.1016/j.plaphy.2013.02.001. View

11.

Yonekura-Sakakibara K, Higashi Y, Nakabayashi R . The Origin and Evolution of Plant Flavonoid Metabolism. Front Plant Sci. 2019; 10:943. PMC: 6688129. DOI: 10.3389/fpls.2019.00943. View

12.

Liao Y, Smyth G, Shi W . featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2013; 30(7):923-30. DOI: 10.1093/bioinformatics/btt656. View

13.

Terrier N, Torregrosa L, Ageorges A, Vialet S, Verries C, Cheynier V . Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in grapevine and suggests additional targets in the pathway. Plant Physiol. 2008; 149(2):1028-41. PMC: 2633825. DOI: 10.1104/pp.108.131862. View

14.

Zhao Q, Yang J, Cui M, Liu J, Fang Y, Yan M . The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis. Mol Plant. 2019; 12(7):935-950. DOI: 10.1016/j.molp.2019.04.002. View

15.

Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, Richard T . Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol. 2005; 140(2):499-511. PMC: 1361319. DOI: 10.1104/pp.105.067231. View

16.

Han S, Xie H, Wang M, Zhang J, Xu Y, Zhu X . Transcriptome and metabolome reveal the effects of three canopy types on the flavonoids and phenolic acids in 'Merlot' (Vitis vinifera L.) berry pericarp. Food Res Int. 2023; 163:112196. DOI: 10.1016/j.foodres.2022.112196. View

17.

Zhao Q, Zhang Y, Wang G, Hill L, Weng J, Chen X . A specialized flavone biosynthetic pathway has evolved in the medicinal plant, Scutellaria baicalensis. Sci Adv. 2016; 2(4):e1501780. PMC: 4846459. DOI: 10.1126/sciadv.1501780. View

18.

Wang N, Liu W, Zhang T, Jiang S, Xu H, Wang Y . Transcriptomic Analysis of Red-Fleshed Apples Reveals the Novel Role of MdWRKY11 in Flavonoid and Anthocyanin Biosynthesis. J Agric Food Chem. 2018; 66(27):7076-7086. DOI: 10.1021/acs.jafc.8b01273. View

19.

Andersen J, Zein I, Wenzel G, Darnhofer B, Eder J, Ouzunova M . Characterization of phenylpropanoid pathway genes within European maize (Zea mays L.) inbreds. BMC Plant Biol. 2008; 8:2. PMC: 2265712. DOI: 10.1186/1471-2229-8-2. View

20.

Wu J, Wang X, Liu Y, Du H, Shu Q, Su S . Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation. Sci Rep. 2016; 6:19245. PMC: 4709722. DOI: 10.1038/srep19245. View