Qualitative Phytochemical Analysis and Assessments of Analgesic and Antidiarrheal Activity of the Methanolic Leaf Extract of Cheilanthes Tenuifolia: In Vivo Study

Overview

Journal Immun Inflamm Dis

Specialties Allergy & Immunology
Infectious Diseases

Date 2024 Nov 7

PMID 39508723

Authors

Asraful Islam Rakib

Raihan Chowdhury

Md Shimul Bhuia

Md Sakib Al Hasan

Salehin Sheikh

Siddique Akber Ansari

Irfan Aamer Ansari

Muhammad Torequl Islam

Affiliations

Soon will be listed here.

Abstract

Background: Cheilanthes tenuifolia (Burm.f.), commonly known as the Sword Fern or Narrow-leaved Cloak Fern, is a little evergreen fern that belongs to the Pteridaceae family and is abundant in various bioactive compounds exerting promising medicinal properties. The current study is designed to evaluate in vivo analgesic and antidiarrheal activity of the methanol leaf extract of C. tenuifolia (MCT).

Methods: For this purpose, Swiss albino mice were used to investigate the analgesic and antidiarrheal properties using acetic acid-induced writhing and castor oil-induced diarrhea methods, respectively. The mice were administered orally with different doses of MCT (125, 250, and 500 mg/kg). The vehicle performed as a negative control (NC), while diclofenac sodium (DCN) (25 mg/kg), loperamide (LOP) (3 mg/kg), bismuth subsalicylate (BSS) (10 mg/kg), and nifedipine (NDP) (2.5 mg/kg) were supplied as positive controls (PC) (p.o.). In both models, combination treatment of MCT (higher dose) and PC was also administered to different groups of animals for assessing potential antagonistic or synergistic activity.

Results: Findings of the in vivo study demonstrated that MCT dose-dependently significantly (p < 0.05) exhibited analgesic and antidiarrheal properties by reduction in the number of writhing and reductions in the total fecal output in contrast to the NC group. Moreover, in combination treatment, MCT significantly (p < 0.05) synergized the activity of PC in both models, exerting potential analgesic and antidiarrheal activity.

Conclusion: In conclusion, MCT has analgesic and antidiarrheal activity; it might be beneficial for the management of pain and diarrhea.

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