Acidification-based Mineral Weathering Mechanism Involves a Glucose/methanol/choline Oxidoreductase in PML1(12)

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2024 Nov 6

PMID 39503492

Authors

Cintia Blanco Nouche

Laura Picard

Carine Cochet

Cedric Paris

Philippe Oger

Marie-Pierre Turpault

Stephane Uroz

Affiliations

Soon will be listed here.

Abstract

Importance: This work deciphers the molecular and genetic bases used by strain PML1(12) of to weather minerals. Through bioinformatics analyses, we identified a total of four GMC-FAD oxidoreductases in the genome of strain PML1(12) and a putative PQQ-dependent glucose dehydrogenase. Through a combination of physiological and geochemical analyses, we revealed that one of them (i.e., GMC3) was the enzyme responsible for the acidification-based mineral weathering mechanism used by strain PML1(12). To date, a single representative of this enzyme family has been identified in the effective mineral-weathering bacterial strain PMB3(1). Phylogenetic analyses revealed that this new system appeared conserved in the genus. The new findings presented in this work demonstrate that GMC oxidoreductases can have an active role in other effective MWe bacteria outside of collimonads and that are capable of weathering minerals using this type of enzyme. Our findings offer relevant information for different fields of research, such as environmental genomics, microbiology, chemistry, evolutionary biology, and soil sciences.

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