Screening and Expression Validation of Key Proteins for Secondary Hair Follicle Growth in Cashmere Goats Based on ITRAQ Quantitative Proteomics Technology

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2024 Oct 30

PMID 39474271

Authors

Jiale Chang

Fanhua Meng

Ru Zhang

Juan Feng

Yujing Liu

Junjie Zhang

Zhaomin Liu

Jiayue Liang

Hongmei Xiao

Affiliations

Soon will be listed here.

Abstract

Background: The growth of secondary hair follicles (SHFs) in cashmere goats has periodic changes, including telogen, anagen, and catagen, during which proteins play important roles as the executor of life activities.

Results: In this study, the skin tissues of cashmere goats at three different growth stages of SHFs were collected for proteome sequencing and validation experiments. Through protein differential expression analysis and time series analysis, FKBP prolyl isomerase 10 (FKBP10) and fibrillin 2 (FBN2) were screened as the key proteins for SHF cycle growth of cashmere goats, and albumin (ALB), collagen type I alpha 1 chain (COL1A1) and elastin (ELN) were predicted to be their interacting proteins. The results of quantitative real-time PCR (qRT-PCR), western blot, and immunohistochemistry experiments showed that the mRNA and protein expression levels of FKBP10, FBN2, COL1A1, ELN and ALB were higher in anagen and lower in telogen. They were all highly expressed in the outer root sheath of SHFs in anagen.

Conclusion: FKBP10, FBN2, COL1A1, ELN, and ALB can promote the growth of SHFs in cashmere goats. This study lays the foundation for analyzing the growth cycle regulatory mechanism of SHFs in cashmere goats, and provides new ideas for further improving cashmere yield and quality.

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