Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2020 Aug 23

PMID 32823629

Citations 10

Authors

Mengli Zhao

Huitong Zhou

Yuzhu Luo

Jiqing Wang

Jiang Hu

Xiu Liu

Shaobin Li

Zhiyun Hao

Xiayang Jin

Yize Song

Xinmiao Wu

Liyan Hu

Jon G H Hickford

Affiliations

Soon will be listed here.

Abstract

Variation in some caprine keratin-associated protein (KAP) genes has been associated with cashmere fiber traits, but many KAP genes remain unidentified in goats. In this study, we confirm the identification of a KAP27-1 gene () and describe its effect on cashmere traits in 248 Longdong cashmere goats. A polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis was used to screen for sequence variation in this gene, and three sequence variants (named to ) were found. These sequences have the highest similarity (77% identity) to a human sequence, while sharing some homology with a predicted caprine sequence ENSCHIG00000023347 in the goat genome construct (ARS1:CM004562.1) at chromosome 1 position 3,966,193-3,973,677 in the forward strand. There were two single nucleotide polymorphisms (SNPs) detected in the coding sequence, including one nonsynonymous SNP (c.413C/T; p.Ala138Val) and one synonymous SNP (c.495C/T). The variant differed from and at c.413C/T, having cytosine in its nucleotide sequence, while the variant differed from and at c.495C/T, having thymine in its nucleotide sequence. Goats of the genotypes and produced cashmere fibers of higher mean fiber diameter (MFD) than goats of genotype , but no difference in MFD was detected between the and goats. These results suggest that is associated with increased MFD. Expression of the caprine sequence was predominantly detected in the skin tissue of goats but not or only weakly detected in other tissues, including longissimus dorsi muscle, heart, kidney, liver, lung and spleen.

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