Evaluation of Anti-biofilm and Anti-virulence Effect of Zinc Sulfate on Staphylococcus Aureus Isolates

Overview

Journal Sci Rep

Specialty Science

Date 2024 Oct 29

PMID 39468094

Authors

Wedad M Abdelraheem

Heba S Kamel

Aya Nabil Gamil

Affiliations

Soon will be listed here.

Abstract

Staphylococcus aureus produces a plethora of virulence factors to invade and establish infections in the host system, and biofilms are more resistant to antibiotics than planktonic cells. In this study, we aimed to investigate the anti-virulence and anti-biofilm potentials of zinc sulfate against S. aureus isolates. The synergistic effect of zinc sulfate in combination with antibiotics on S. aureus was characterized using the checkerboard method. The influence of zinc sulfate on biofilm formation and virulence factors production by S. aureus was experimentally assessed. RT-qPCR was used to investigate the effect of zinc sulfate on the expression of biofilm-related genes. Zinc sulfate exhibited good antibacterial activity against S. aureus with a MIC of 128 µg/ml against all tested isolates. Also, the findings indicate a synergistic effect of a combination of zinc sulfate and antibiotics against the tested isolates. Zinc sulfate at 256 µg/ml concentration inhibited biofilm formation for all isolates. The expression of biofilm-related genes was significantly repressed in zinc sulfate-treated bacteria compared to untreated cells. Zinc sulfate could inhibit the hemolytic ability of S. aureus. Moreover, zinc sulfate-treated bacteria exhibited a significant decrease in coagulase and catalase activity relative to control untreated S. aureus. Our results support that zinc sulfate is a potential antimicrobial and anti-virulence agent against S. aureus infections.

References

Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I . Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8):891-9. DOI: 10.1111/j.1600-0463.2007.apm_630.x. View

Almoudi M, Hussein A, Abu Hassan M, Zain N . A systematic review on antibacterial activity of zinc against . Saudi Dent J. 2018; 30(4):283-291. PMC: 6128804. DOI: 10.1016/j.sdentj.2018.06.003. View

Almuhayawi M, Alruhaili M, Gattan H, Alharbi M, Nagshabandi M, Al Jaouni S . Induced Wound Infections Which Antimicrobial Resistance, Methicillin- and Vancomycin-Resistant: Assessment of Emergence and Cross Sectional Study. Infect Drug Resist. 2023; 16:5335-5346. PMC: 10440082. DOI: 10.2147/IDR.S418681. View

Saeed A, Ahsan F, Nawaz M, Iqbal K, Rehman K, Ijaz T . Incidence of Vancomycin Resistant Phenotype of the Methicillin Resistant Isolated from a Tertiary Care Hospital in Lahore. Antibiotics (Basel). 2019; 9(1). PMC: 7168916. DOI: 10.3390/antibiotics9010003. View

Prakash P, Rajan V, Gopal S . Predominance of SCCmec types IV and V among biofilm producing device-associated Staphylococcus aureus strains isolated from tertiary care hospitals in Mysuru, India. Enferm Infecc Microbiol Clin. 2016; 35(4):229-235. DOI: 10.1016/j.eimc.2016.09.005. View

Bimanand L, Taherikalani M, Jalilian F, Sadeghifard N, Ghafourian S, Mahdavi Z . Association between biofilm production, adhesion genes and drugs resistance in different SCCmec types of methicillin resistant strains isolated from several major hospitals of Iran. Iran J Basic Med Sci. 2018; 21(4):400-403. PMC: 5960757. DOI: 10.22038/IJBMS.2018.19378.5132. View

Phan T, Buckner T, Sheng J, Baldeck J, Marquis R . Physiologic actions of zinc related to inhibition of acid and alkali production by oral streptococci in suspensions and biofilms. Oral Microbiol Immunol. 2003; 19(1):31-8. DOI: 10.1046/j.0902-0055.2003.00109.x. View

Pati R, Mehta R, Mohanty S, Padhi A, Sengupta M, Vaseeharan B . Topical application of zinc oxide nanoparticles reduces bacterial skin infection in mice and exhibits antibacterial activity by inducing oxidative stress response and cell membrane disintegration in macrophages. Nanomedicine. 2014; 10(6):1195-208. DOI: 10.1016/j.nano.2014.02.012. View

Neopane P, Nepal H, Shrestha R, Uehara O, Abiko Y . In vitro biofilm formation by isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance. Int J Gen Med. 2018; 11:25-32. PMC: 5779313. DOI: 10.2147/IJGM.S153268. View

10.

Abdelraheem W, Khairy R, Zaki A, Zaki S . Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates. Ann Clin Microbiol Antimicrob. 2021; 20(1):54. PMC: 8379777. DOI: 10.1186/s12941-021-00459-2. View

11.

Bellio P, Fagnani L, Nazzicone L, Celenza G . New and simplified method for drug combination studies by checkerboard assay. MethodsX. 2021; 8:101543. PMC: 8563647. DOI: 10.1016/j.mex.2021.101543. View

12.

Sedarat Z, Taylor-Robinson A . Biofilm Formation by Pathogenic Bacteria: Applying a Model to Appraise Potential Targets for Therapeutic Intervention. Pathogens. 2022; 11(4). PMC: 9027693. DOI: 10.3390/pathogens11040388. View

13.

Roohani N, Hurrell R, Kelishadi R, Schulin R . Zinc and its importance for human health: An integrative review. J Res Med Sci. 2013; 18(2):144-57. PMC: 3724376. View

14.

Fatima T, Haji Abdul Rahim Z, Lin C, Qamar Z . Zinc: A precious trace element for oral health care?. J Pak Med Assoc. 2016; 66(8):1019-23. View

15.

Tsige Y, Tadesse S, GEyesus T, Tefera M, Amsalu A, Menberu M . Prevalence of Methicillin-Resistant and Associated Risk Factors among Patients with Wound Infection at Referral Hospital, Northeast Ethiopia. J Pathog. 2020; 2020:3168325. PMC: 7271240. DOI: 10.1155/2020/3168325. View

16.

Sahal G, Woerdenbag H, Hinrichs W, Visser A, Tepper P, Quax W . Antifungal and biofilm inhibitory effect of Cymbopogon citratus (lemongrass) essential oil on biofilm forming by Candida tropicalis isolates; an in vitro study. J Ethnopharmacol. 2019; 246:112188. DOI: 10.1016/j.jep.2019.112188. View

17.

Ahmed E, Rasmi A, Darwish A, Gad G . Prevalence and resistance profile of bacteria isolated from wound infections among a group of patients in upper Egypt: a descriptive cross-sectional study. BMC Res Notes. 2023; 16(1):106. PMC: 10280819. DOI: 10.1186/s13104-023-06379-y. View

18.

Lister J, Horswill A . Staphylococcus aureus biofilms: recent developments in biofilm dispersal. Front Cell Infect Microbiol. 2015; 4:178. PMC: 4275032. DOI: 10.3389/fcimb.2014.00178. View

19.

Abdelghafar A, Yousef N, Askoura M . Zinc oxide nanoparticles reduce biofilm formation, synergize antibiotics action and attenuate Staphylococcus aureus virulence in host; an important message to clinicians. BMC Microbiol. 2022; 22(1):244. PMC: 9552502. DOI: 10.1186/s12866-022-02658-z. View

20.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View