Staphylococcus Aureus Biofilms: Recent Developments in Biofilm Dispersal

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2015 Jan 8

PMID 25566513

Citations 263

Authors

Jessica L Lister

Alexander R Horswill

Affiliations

Soon will be listed here.

Abstract

Staphylococcus aureus is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The formation of a biofilm, and encasement of cells in a polymer-based matrix, decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate. During infection, dispersal of cells from the biofilm can result in spread to secondary sites and worsening of the infection. In this review, we discuss the current understanding of the pathways behind biofilm dispersal in S. aureus, with a focus on enzymatic and newly described broad-spectrum dispersal mechanisms. Additionally, we explore potential applications of dispersal in the treatment of biofilm-mediated infections.

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References

Sanchez Jr C, Prieto E, Krueger C, Zienkiewicz K, Romano D, Ward C . Effects of local delivery of D-amino acids from biofilm-dispersive scaffolds on infection in contaminated rat segmental defects. Biomaterials. 2013; 34(30):7533-43. DOI: 10.1016/j.biomaterials.2013.06.026. View

Kolar S, Ibarra J, Rivera F, Mootz J, Davenport J, Stevens S . Extracellular proteases are key mediators of Staphylococcus aureus virulence via the global modulation of virulence-determinant stability. Microbiologyopen. 2012; 2(1):18-34. PMC: 3584211. DOI: 10.1002/mbo3.55. View

Cheung A, Fischetti V . The role of fibrinogen in staphylococcal adherence to catheters in vitro. J Infect Dis. 1990; 161(6):1177-86. DOI: 10.1093/infdis/161.6.1177. View

Toledo-Arana A, Merino N, Vergara-Irigaray M, Debarbouille M, Penades J, Lasa I . Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system. J Bacteriol. 2005; 187(15):5318-29. PMC: 1196035. DOI: 10.1128/JB.187.15.5318-5329.2005. View

Foster T, Geoghegan J, Ganesh V, Hook M . Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol. 2013; 12(1):49-62. PMC: 5708296. DOI: 10.1038/nrmicro3161. View

Novotny L, Amer A, Brockson M, Goodman S, Bakaletz L . Structural stability of Burkholderia cenocepacia biofilms is reliant on eDNA structure and presence of a bacterial nucleic acid binding protein. PLoS One. 2013; 8(6):e67629. PMC: 3682984. DOI: 10.1371/journal.pone.0067629. View

ONeill E, Pozzi C, Houston P, Smyth D, Humphreys H, Robinson D . Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol. 2007; 45(5):1379-88. PMC: 1865887. DOI: 10.1128/JCM.02280-06. View

Darouiche R . Treatment of infections associated with surgical implants. N Engl J Med. 2004; 350(14):1422-9. DOI: 10.1056/NEJMra035415. View

OGara J . ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus. FEMS Microbiol Lett. 2007; 270(2):179-88. DOI: 10.1111/j.1574-6968.2007.00688.x. View

10.

Mann E, Rice K, Boles B, Endres J, Ranjit D, Chandramohan L . Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation. PLoS One. 2009; 4(6):e5822. PMC: 2688759. DOI: 10.1371/journal.pone.0005822. View

11.

Parsek M, Singh P . Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003; 57:677-701. DOI: 10.1146/annurev.micro.57.030502.090720. View

12.

Lauderdale K, Malone C, Boles B, Morcuende J, Horswill A . Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material. J Orthop Res. 2009; 28(1):55-61. DOI: 10.1002/jor.20943. View

13.

Costerton J, Stewart P, Greenberg E . Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418):1318-22. DOI: 10.1126/science.284.5418.1318. View

14.

Goodman S, Obergfell K, Jurcisek J, Novotny L, Downey J, Ayala E . Biofilms can be dispersed by focusing the immune system on a common family of bacterial nucleoid-associated proteins. Mucosal Immunol. 2011; 4(6):625-37. DOI: 10.1038/mi.2011.27. View

15.

Chatterjee S, Maiti P, Dey R, Kundu A, Dey R . Biofilms on indwelling urologic devices: microbes and antimicrobial management prospect. Ann Med Health Sci Res. 2014; 4(1):100-4. PMC: 3952279. DOI: 10.4103/2141-9248.126612. View

16.

Kaplan J, LoVetri K, Cardona S, Madhyastha S, Sadovskaya I, Jabbouri S . Recombinant human DNase I decreases biofilm and increases antimicrobial susceptibility in staphylococci. J Antibiot (Tokyo). 2011; 65(2):73-7. PMC: 3288126. DOI: 10.1038/ja.2011.113. View

17.

Conlon B, Nakayasu E, Fleck L, LaFleur M, Isabella V, Coleman K . Activated ClpP kills persisters and eradicates a chronic biofilm infection. Nature. 2013; 503(7476):365-70. PMC: 4031760. DOI: 10.1038/nature12790. View

18.

Huseby M, Kruse A, Digre J, Kohler P, Vocke J, Mann E . Beta toxin catalyzes formation of nucleoprotein matrix in staphylococcal biofilms. Proc Natl Acad Sci U S A. 2010; 107(32):14407-12. PMC: 2922554. DOI: 10.1073/pnas.0911032107. View

19.

Rohde H, Burandt E, Siemssen N, Frommelt L, Burdelski C, Wurster S . Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections. Biomaterials. 2006; 28(9):1711-20. DOI: 10.1016/j.biomaterials.2006.11.046. View

20.

Hu L, Umeda A, Kondo S, Amako K . Typing of Staphylococcus aureus colonising human nasal carriers by pulsed-field gel electrophoresis. J Med Microbiol. 1995; 42(2):127-32. DOI: 10.1099/00222615-42-2-127. View