Hypoxia Modulates Sodium Chloride Co-transporter Via CaMKII-β Pathway: An In Vitro Study with MDCT15 Cells

Overview

Journal Life (Basel)

Specialty Biology

Date 2024 Oct 26

PMID 39459529

Authors

Lijuan Liang

Kohei Ueda

Sayoko Ogura

Tatsuo Shimosawa

Affiliations

Soon will be listed here.

Abstract

Background: Hypoxia plays a crucial role in regulating various cellular functions, including ion-transport mechanisms in the kidney. The sodium-chloride co-transporter (NCC) is essential for sodium reabsorption in the distal convoluted tubule (DCT). However, the effects of hypoxia on NCC expression and its regulatory pathways remain unclear. We aimed to explore the effects and potential mechanisms of hypoxia on NCC in vitro.

Methods: mDCT15 cells were treated with cobalt chloride (CoCl) at a concentration of 300 μmol/L to induce hypoxia. The cells were harvested at different time points, namely 30 min, 1 h, 6 h, and 24 h, and the expression of NCC and CaMKII-β was analyzed using Western blot.

Results: A time-dependent upregulation of NCC and CaMKII-β expression in response to CoCl-induced hypoxia. KN93 reversed the effect of CoCl on NCC and phosphorylated NCC expression.

Conclusions: Hypoxia, mediated through cobalt chloride treatment, upregulates NCC expression via the CaMKII-β pathway in mDCT15 cells.

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