Optimizing Diagnosis and Management of Ventilator-Associated Pneumonia: A Systematic Evaluation of Biofilm Detection Methods and Bacterial Colonization on Endotracheal Tubes

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Oct 26

PMID 39458275

Authors

Ioana Roxana Codru

Bogdan Ioan Vintila

Mihai Sava

Alina Simona Bereanu

Sandra Ioana Neamtu

Raluca Maria Badila

Victoria Birlutiu

Affiliations

Soon will be listed here.

Abstract

Healthcare-associated infections, such as ventilator-associated pneumonia and biofilm formation on intubation cannulas, impose significant burdens on hospitals, affecting staffing, finances, and patient wellbeing, while also increasing the risk of patient mortality. We propose a research study aimed at exploring various methodologies for detecting these infections, discovered in the biofilm on medical devices, particularly tracheal cannulas, and understanding the role of each method in comprehending these infections from an etiological perspective. Our investigation also involves an analysis of the types of endotracheal tubes utilized in each case, the bacteria species identified, and strategies for combating biofilm-associated infections. The potential impact of our research is the substantial improvement of patient care through enhanced diagnosis and management of these infections.

Citing Articles

Antimicrobial Resistance Patterns and Biofilm Analysis via Sonication in Intensive Care Unit Patients at a County Emergency Hospital in Romania.

Codru I, Vintila B, Bereanu A, Sava M, Popa L, Birlutiu V Pharmaceuticals (Basel). 2025; 18(2).

PMID: 40005975 PMC: 11858300. DOI: 10.3390/ph18020161.

References

Shaqour B, Aizawa J, Guarch-Perez C, Gorecka Z, Christophersen L, Martinet W . Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model. Pharmaceutics. 2021; 13(6). PMC: 8224298. DOI: 10.3390/pharmaceutics13060772. View

Boicean A, Neamtu B, Birsan S, Batar F, Tanasescu C, Dura H . Fecal Microbiota Transplantation in Patients Co-Infected with SARS-CoV2 and . Biomedicines. 2023; 11(1). PMC: 9855959. DOI: 10.3390/biomedicines11010007. View

Birlutiu R, Birlutiu V, Cismasiu R, Mihalache M . bbFISH-ing in the sonication fluid. Medicine (Baltimore). 2019; 98(29):e16501. PMC: 6709156. DOI: 10.1097/MD.0000000000016501. View

Kiarostami K, Fernandez-Barat L, Battaglini D, Motos A, Bueno-Freire L, Soler-Comas A . The efficacy of telavancin in comparison with linezolid on endotracheal tube biofilm in pigs with methicillin-resistant Staphylococcus aureus pneumonia. Int J Antimicrob Agents. 2023; 63(2):107052. DOI: 10.1016/j.ijantimicag.2023.107052. View

Sharma S, Mohler J, Mahajan S, Schwartz S, Bruggemann L, Aalinkeel R . Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment. Microorganisms. 2023; 11(6). PMC: 10305407. DOI: 10.3390/microorganisms11061614. View

Silveira G, Torres M, Ribeiro C, Meneguetti B, Carvalho C, de la Fuente-Nunez C . Antibiofilm Peptides: Relevant Preclinical Animal Infection Models and Translational Potential. ACS Pharmacol Transl Sci. 2021; 4(1):55-73. PMC: 7887742. DOI: 10.1021/acsptsci.0c00191. View

Sauer K, Stoodley P, Goeres D, Hall-Stoodley L, Burmolle M, Stewart P . The biofilm life cycle: expanding the conceptual model of biofilm formation. Nat Rev Microbiol. 2022; 20(10):608-620. PMC: 9841534. DOI: 10.1038/s41579-022-00767-0. View

Lethongkam S, Sunghan J, Wangdee C, Durongphongtorn S, Siri R, Wunnoo S . Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital. Appl Microbiol Biotechnol. 2022; 107(2-3):623-638. PMC: 9780629. DOI: 10.1007/s00253-022-12327-w. View

Oliveira V, Macedo A, Melo L, Santos S, Hermann P, Silva-Lovato C . Bacteriophage Cocktail-Mediated Inhibition of Biofilm on Endotracheal Tube Surface. Antibiotics (Basel). 2021; 10(1). PMC: 7830274. DOI: 10.3390/antibiotics10010078. View

10.

van Charante F, Wieme A, Rigole P, De Canck E, Ostyn L, Grassi L . Microbial diversity and antimicrobial susceptibility in endotracheal tube biofilms recovered from mechanically ventilated COVID-19 patients. Biofilm. 2022; 4:100079. PMC: 9192360. DOI: 10.1016/j.bioflm.2022.100079. View

11.

Alves D, Grainha T, Pereira M, Lopes S . Antimicrobial materials for endotracheal tubes: A review on the last two decades of technological progress. Acta Biomater. 2023; 158:32-55. DOI: 10.1016/j.actbio.2023.01.001. View

12.

Garcia-Vaquero M, Rajauria G, ODoherty J, Sweeney T . Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification. Food Res Int. 2017; 99(Pt 3):1011-1020. DOI: 10.1016/j.foodres.2016.11.016. View

13.

Daengngam C, Lethongkam S, Srisamran P, Paosen S, Wintachai P, Anantravanit B . Green fabrication of anti-bacterial biofilm layer on endotracheal tubing using silver nanoparticles embedded in polyelectrolyte multilayered film. Mater Sci Eng C Mater Biol Appl. 2019; 101:53-63. DOI: 10.1016/j.msec.2019.03.061. View

14.

Oliveira V, Bim F, Monteiro R, Macedo A, Santos E, Silva-Lovato C . Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Biofilm on Endotracheal Tubes. Front Microbiol. 2020; 11:580779. PMC: 7573221. DOI: 10.3389/fmicb.2020.580779. View

15.

Birlutiu R, Roman M, Cismasiu R, Fleaca S, Popa C, Mihalache M . Sonication contribution to identifying prosthetic joint infection with Ralstonia pickettii: a case report and review of the literature. BMC Musculoskelet Disord. 2017; 18(1):311. PMC: 5518162. DOI: 10.1186/s12891-017-1678-y. View

16.

Zangirolami A, Dias L, Blanco K, Vinagreiro C, Inada N, Arnaut L . Avoiding ventilator-associated pneumonia: Curcumin-functionalized endotracheal tube and photodynamic action. Proc Natl Acad Sci U S A. 2020; 117(37):22967-22973. PMC: 7502737. DOI: 10.1073/pnas.2006759117. View

17.

Birlutiu V, Birlutiu R . Endocarditis due to Abiotrophia defectiva, a biofilm-related infection associated with the presence of fixed braces: A case report. Medicine (Baltimore). 2017; 96(46):e8756. PMC: 5704873. DOI: 10.1097/MD.0000000000008756. View

18.

Luo Y, McAuley D, Fulton C, Sa Pessoa J, McMullan R, Lundy F . Targeting Candida albicans in dual-species biofilms with antifungal treatment reduces Staphylococcus aureus and MRSA in vitro. PLoS One. 2021; 16(4):e0249547. PMC: 8031443. DOI: 10.1371/journal.pone.0249547. View

19.

Rather M, Gupta K, Mandal M . Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies. Braz J Microbiol. 2021; 52(4):1701-1718. PMC: 8578483. DOI: 10.1007/s42770-021-00624-x. View

20.

Rao H, Choo S, Rajeswari Mahalingam S, Adisuri D, Madhavan P, Md Akim A . Approaches for Mitigating Microbial Biofilm-Related Drug Resistance: A Focus on Micro- and Nanotechnologies. Molecules. 2021; 26(7). PMC: 8036581. DOI: 10.3390/molecules26071870. View