Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Biofilm on Endotracheal Tubes

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2020 Oct 30

PMID 33123112

Citations 23

Authors

Viviane C Oliveira

Felipe L Bim

Rachel M Monteiro

Ana Paula Macedo

Emerson S Santos

Claudia H Silva-Lovato

Helena F O Paranhos

Luis D R Melo

Silvio B Santos

Evandro Watanabe

Affiliations

Soon will be listed here.

Abstract

Studies involving antimicrobial-coated endotracheal tubes are scarce, and new approaches to control multidrug-resistant biofilm on these devices should be investigated. In this study, five new bacteriophages from domestic sewage were isolated. All of them belong to the order , family. They are pH and heat stable and produce 27 to 46 particles after a latent period of 30 min at 37°C. Their dsDNA genome (ranging from ∼62 to ∼65 kb) encodes 65 to 89 different putative proteins. They exhibit a broad lytic spectrum and infect 69.7% of the strains tested. All the bacteriophages were able to reduce the growth of strains in planktonic form. The bacteriophages were also able to reduce the biofilm viability rates and the metabolic activity of strains in a model of biofilms associated with endotracheal tubes. In addition, scanning electron microscopy micrographs showed disrupted biofilms and cell debris after treatment of bacteriophages, revealing remarkable biofilm reduction. The lytic activity on multidrug-resistant biofilm indicates that the isolated bacteriophages might be considered as good candidates for therapeutic studies and for the application of bacteriophage-encoded products.

Citing Articles

Isolation and Characterization of Lytic Bacteriophages Capable of Infecting Diverse Multidrug-Resistant Strains of : PaCCP1 and PaCCP2.

Parra B, Sandoval M, Arriagada V, Amsteins L, Aguayo C, Opazo-Capurro A Pharmaceuticals (Basel). 2025; 17(12.

PMID: 39770458 PMC: 11728774. DOI: 10.3390/ph17121616.

Biofilm Production in Intensive Care Units: Challenges and Implications.

Taner F, Baddal B, Theodoridis L, Petrovski S Pathogens. 2024; 13(11).

PMID: 39599508 PMC: 11597785. DOI: 10.3390/pathogens13110954.

The synergistic effect between phages and Ceftolozane/Tazobactam in endotracheal tube biofilm.

Oliveira V, Soler-Comas A, Rocha A, Silva-Lovato C, Watanabe E, Torres A Emerg Microbes Infect. 2024; 13(1):2420737.

PMID: 39530158 PMC: 11571741. DOI: 10.1080/22221751.2024.2420737.

Isolation and preliminary characterization of a novel bacteriophage vB_KquU_φKuK6 that infects the multidrug-resistant pathogen .

Miller I, Laney A, Zahn G, Sheehan B, Whitley K, Kuddus R Front Microbiol. 2024; 15:1472729.

PMID: 39479209 PMC: 11524547. DOI: 10.3389/fmicb.2024.1472729.

Optimizing Diagnosis and Management of Ventilator-Associated Pneumonia: A Systematic Evaluation of Biofilm Detection Methods and Bacterial Colonization on Endotracheal Tubes.

Codru I, Vintila B, Sava M, Bereanu A, Neamtu S, Badila R Microorganisms. 2024; 12(10).

PMID: 39458275 PMC: 11509713. DOI: 10.3390/microorganisms12101966.

References

Schneider L, Korber A, Grabbe S, Dissemond J . Influence of pH on wound-healing: a new perspective for wound-therapy?. Arch Dermatol Res. 2006; 298(9):413-20. DOI: 10.1007/s00403-006-0713-x. View

Soding J . Protein homology detection by HMM-HMM comparison. Bioinformatics. 2004; 21(7):951-60. DOI: 10.1093/bioinformatics/bti125. View

Schattner P, Brooks A, Lowe T . The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res. 2005; 33(Web Server issue):W686-9. PMC: 1160127. DOI: 10.1093/nar/gki366. View

Lessa F, Fabio Aranha A, Nogueira I, Giro E, Hebling J, de Souza Costa C . Toxicity of chlorhexidine on odontoblast-like cells. J Appl Oral Sci. 2010; 18(1):50-8. PMC: 5349028. DOI: 10.1590/s1678-77572010000100010. View

Petersen T, Brunak S, von Heijne G, Nielsen H . SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods. 2011; 8(10):785-6. DOI: 10.1038/nmeth.1701. View

Melo L, Veiga P, Cerca N, Kropinski A, Almeida C, Azeredo J . Development of a Phage Cocktail to Control Proteus mirabilis Catheter-associated Urinary Tract Infections. Front Microbiol. 2016; 7:1024. PMC: 4923195. DOI: 10.3389/fmicb.2016.01024. View

Tokmaji G, Vermeulen H, Muller M, Kwakman P, Schultz M, Zaat S . Silver-coated endotracheal tubes for prevention of ventilator-associated pneumonia in critically ill patients. Cochrane Database Syst Rev. 2015; (8):CD009201. PMC: 6517140. DOI: 10.1002/14651858.CD009201.pub2. View

Akturk E, Oliveira H, Santos S, Costa S, Kuyumcu S, Melo L . Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms. Antibiotics (Basel). 2019; 8(3). PMC: 6783858. DOI: 10.3390/antibiotics8030103. View

Haas C, Eakin R, Konkle M, Blank R . Endotracheal tubes: old and new. Respir Care. 2014; 59(6):933-52. DOI: 10.4187/respcare.02868. View

10.

Cui Z, Feng T, Gu F, Li Q, Dong K, Zhang Y . Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China. Virol J. 2017; 14(1):26. PMC: 5299689. DOI: 10.1186/s12985-017-0701-0. View

11.

Mao B, Chen Z, Wang Y, Yan S . Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses. Sci Rep. 2018; 8(1):2445. PMC: 5799281. DOI: 10.1038/s41598-018-20728-z. View

12.

Nzakizwanayo J, Hanin A, Alves D, McCutcheon B, Dedi C, Salvage J . Bacteriophage Can Prevent Encrustation and Blockage of Urinary Catheters by Proteus mirabilis. Antimicrob Agents Chemother. 2015; 60(3):1530-6. PMC: 4775969. DOI: 10.1128/AAC.02685-15. View

13.

Cieplik F, Jakubovics N, Buchalla W, Maisch T, Hellwig E, Al-Ahmad A . Resistance Toward Chlorhexidine in Oral Bacteria - Is There Cause for Concern?. Front Microbiol. 2019; 10:587. PMC: 6439480. DOI: 10.3389/fmicb.2019.00587. View

14.

Forti F, Roach D, Cafora M, Pasini M, Horner D, Fiscarelli E . Design of a Broad-Range Bacteriophage Cocktail That Reduces Pseudomonas aeruginosa Biofilms and Treats Acute Infections in Two Animal Models. Antimicrob Agents Chemother. 2018; 62(6). PMC: 5971607. DOI: 10.1128/AAC.02573-17. View

15.

Finn R, Attwood T, Babbitt P, Bateman A, Bork P, Bridge A . InterPro in 2017-beyond protein family and domain annotations. Nucleic Acids Res. 2016; 45(D1):D190-D199. PMC: 5210578. DOI: 10.1093/nar/gkw1107. View

16.

Aleshkin A, Ershova O, Volozhantsev N, Svetoch E, Popova A, Rubalskii E . Phagebiotics in treatment and prophylaxis of healthcare-associated infections. Bacteriophage. 2017; 6(4):e1251379. PMC: 5221749. DOI: 10.1080/21597081.2016.1251379. View

17.

Spilker T, Coenye T, Vandamme P, LiPuma J . PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. J Clin Microbiol. 2004; 42(5):2074-9. PMC: 404678. DOI: 10.1128/JCM.42.5.2074-2079.2004. View

18.

Lin D, Koskella B, Lin H . Phage therapy: An alternative to antibiotics in the age of multi-drug resistance. World J Gastrointest Pharmacol Ther. 2017; 8(3):162-173. PMC: 5547374. DOI: 10.4292/wjgpt.v8.i3.162. View

19.

Barros J, Melo L, Silva R, Ferraz M, Azeredo J, Pinheiro V . Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine. 2019; 24:102145. DOI: 10.1016/j.nano.2019.102145. View

20.

Nseir S, Ader F, Lubret R, Marquette C . Pathophysiology of airway colonization in critically ill COPD patient. Curr Drug Targets. 2011; 12(4):514-20. DOI: 10.2174/138945011794751537. View