Volatile Organic Compounds Produced by Co-Culture of B418 with T11-W Exhibits Improved Antagonistic Activities Against Fungal Phytopathogens

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Oct 26

PMID 39456879

Authors

Wenzhe Li

Xinyue Wang

Yanqing Jiang

Shuning Cui

Jindong Hu

Yanli Wei

Jishun Li

Yuanzheng Wu

Affiliations

Soon will be listed here.

Abstract

Recently, there has been a growing interest in the biocontrol activity of volatile organic compounds (VOCs) produced by microorganisms. This study specifically focuses on the effects of VOCs produced by the co-culture of B418 and T11-W for the control of two phytopathogenic fungi, and f. sp. Owen. The antagonistic activity of VOCs released in mono- and co-culture modes was evaluated by inhibition assays on a Petri dish and in detached fruit experiments, with the co-culture demonstrating significantly higher inhibitory effects on the phytopathogens on both the plates and fruits compared with the mono-cultures. Metabolomic profiles of VOCs were conducted using the solid-liquid microextraction technique, revealing 341 compounds with significant changes in their production during the co-culture. Among these compounds, linalool, dimethyl trisulfide, dimethyl disulfide, geranylacetone, 2-phenylethanol, and acetophenone were identified as having strong antagonistic activity through a standard inhibition assay. These key compounds were found to be related to the improved inhibitory effect of the B418 and T11-W co-culture. Overall, the results suggest that VOCs produced by the co-culture of B418 and T11-W possess great potential in biological control.

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PMID: 39567403 DOI: 10.1007/s00203-024-04199-7.

References

Tran T, Olsson M, McMillan D, Cullen J, Parsons P, Reddell P . Potent Antibacterial Prenylated Acetophenones from the Australian Endemic Plant . Antibiotics (Basel). 2020; 9(8). PMC: 7460405. DOI: 10.3390/antibiotics9080487. View

Schroeckh V, Scherlach K, Nutzmann H, Shelest E, Schmidt-Heck W, Schuemann J . Intimate bacterial-fungal interaction triggers biosynthesis of archetypal polyketides in Aspergillus nidulans. Proc Natl Acad Sci U S A. 2009; 106(34):14558-63. PMC: 2732885. DOI: 10.1073/pnas.0901870106. View

Effmert U, Kalderas J, Warnke R, Piechulla B . Volatile mediated interactions between bacteria and fungi in the soil. J Chem Ecol. 2012; 38(6):665-703. DOI: 10.1007/s10886-012-0135-5. View

Snelders N, Petti G, van den Berg G, Seidl M, Thomma B . An ancient antimicrobial protein co-opted by a fungal plant pathogen for in planta mycobiome manipulation. Proc Natl Acad Sci U S A. 2021; 118(49). PMC: 8670511. DOI: 10.1073/pnas.2110968118. View

Scherlach K, Graupner K, Hertweck C . Molecular bacteria-fungi interactions: effects on environment, food, and medicine. Annu Rev Microbiol. 2013; 67:375-97. DOI: 10.1146/annurev-micro-092412-155702. View

Zou X, Wei Y, Jiang S, Xu F, Wang H, Zhan P . ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against . J Agric Food Chem. 2022; 70(45):14468-14479. DOI: 10.1021/acs.jafc.2c06187. View

Kumar N, Hoque M, Sugimoto M . Robust volcano plot: identification of differential metabolites in the presence of outliers. BMC Bioinformatics. 2018; 19(1):128. PMC: 5896081. DOI: 10.1186/s12859-018-2117-2. View

Guo R, Ji S, Wang Z, Zhang H, Wang Y, Liu Z . Trichoderma asperellum xylanases promote growth and induce resistance in poplar. Microbiol Res. 2021; 248:126767. DOI: 10.1016/j.micres.2021.126767. View

Das P, Effmert U, Baermann G, Quella M, Piechulla B . Impact of bacterial volatiles on phytopathogenic fungi: an in vitro study on microbial competition and interaction. J Exp Bot. 2021; 73(2):596-614. DOI: 10.1093/jxb/erab476. View

10.

Bitas V, Kim H, Bennett J, Kang S . Sniffing on microbes: diverse roles of microbial volatile organic compounds in plant health. Mol Plant Microbe Interact. 2013; 26(8):835-43. DOI: 10.1094/MPMI-10-12-0249-CR. View

11.

Chen J, Xiang W, Cao K, Lu X, Yao S, Hung D . Characterization of Volatile Organic Compounds Emitted from Endophytic ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens. Molecules. 2020; 25(17). PMC: 7504634. DOI: 10.3390/molecules25173765. View

12.

Carrion V, Cordovez V, Tyc O, Etalo D, de Bruijn I, de Jager V . Involvement of Burkholderiaceae and sulfurous volatiles in disease-suppressive soils. ISME J. 2018; 12(9):2307-2321. PMC: 6092406. DOI: 10.1038/s41396-018-0186-x. View

13.

Tyagi S, Lee K, Shukla P, Chae J . Dimethyl disulfide exerts antifungal activity against Sclerotinia minor by damaging its membrane and induces systemic resistance in host plants. Sci Rep. 2020; 10(1):6547. PMC: 7162885. DOI: 10.1038/s41598-020-63382-0. View

14.

Schmidt R, Cordovez V, de Boer W, Raaijmakers J, Garbeva P . Volatile affairs in microbial interactions. ISME J. 2015; 9(11):2329-35. PMC: 4611499. DOI: 10.1038/ismej.2015.42. View

15.

Lemfack M, Gohlke B, Toguem S, Preissner S, Piechulla B, Preissner R . mVOC 2.0: a database of microbial volatiles. Nucleic Acids Res. 2017; 46(D1):D1261-D1265. PMC: 5753297. DOI: 10.1093/nar/gkx1016. View

16.

Dudareva N, Klempien A, Muhlemann J, Kaplan I . Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytol. 2013; 198(1):16-32. DOI: 10.1111/nph.12145. View

17.

Gamalero E, Glick B . The Use of Plant Growth-Promoting Bacteria to Prevent Nematode Damage to Plants. Biology (Basel). 2020; 9(11). PMC: 7695023. DOI: 10.3390/biology9110381. View

18.

Dandurishvili N, Toklikishvili N, Ovadis M, Eliashvili P, Giorgobiani N, Keshelava R . Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants. J Appl Microbiol. 2010; 110(1):341-52. DOI: 10.1111/j.1365-2672.2010.04891.x. View

19.

Mironenka J, Rozalska S, Sobon A, Bernat P . Trichoderma harzianum metabolites disturb Fusarium culmorum metabolism: Metabolomic and proteomic studies. Microbiol Res. 2021; 249:126770. DOI: 10.1016/j.micres.2021.126770. View

20.

Du F, Ju G, Xiao L, Zhou Y, Wu X . Sesquiterpenes and Cyclodepsipeptides from Marine-Derived Fungus and Their Antagonistic Activities against Soil-borne Pathogens. Mar Drugs. 2020; 18(3). PMC: 7142749. DOI: 10.3390/md18030165. View