Characterization of Volatile Organic Compounds Emitted from Endophytic ETR-B22 by SPME-GC-MS and Their Inhibitory Activity Against Various Plant Fungal Pathogens

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Aug 23

PMID 32824884

Citations 14

Authors

Jian-Hua Chen

Wei Xiang

Ke-Xin Cao

Xuan Lu

Shao-Chang Yao

Ding Hung

Rong-Shao Huang

Liang-Bo Li

Affiliations

Soon will be listed here.

Abstract

The use of antagonistic microorganisms and their volatile organic compounds (VOCs) to control plant fungal pathogens is an eco-friendly and promising substitute for chemical fungicides. In this work, endophytic bacterium ETR-B22, isolated from the root of Gagnep., was found to exhibit strong antagonistic activity against 12 fungal pathogens found in agriculture. Strain ETR-B22 was identified as based on 16S rRNA and sequences. We evaluated the antifungal activity of VOCs emitted by ETR-B22. The VOCs from strain ETR-B22 also showed broad-spectrum antifungal activity against 12 fungal pathogens. The composition of the volatile profiles was analyzed based on headspace solid phase microextraction (HS-SPME) gas chromatography coupled to mass spectrometry (GC-MS). Different extraction strategies for the SPME process significantly affected the extraction efficiency of the VOCs. Thirty-two different VOCs were identified. Among the VOC of ETR-B22, dimethyl trisulfide, indole, methyl anthranilate, methyl salicylate, methyl benzoate, benzyl propionate, benzyl acetate, 3,5-di-tert-butylphenol, allyl benzyl ether and nonanoic acid showed broad-spectrum antifungal activity, and are key inhibitory compounds produced by strain ETR-B22 against various fungal pathogens. Our results suggest that the endophytic strain ETR-B22 and its VOCs have high potential for use as biological controls of plant fungal pathogens.

Citing Articles

Actinobacteria Warfare Against the Plant Pathogen Sclerotinia sclerotiorum: 2,4,6-Trimethylpyridine Identified as a Bacterial Derived Volatile With Antifungal Activity.

Belt K, Flematti G, Bohman B, Chooi H, Roper M, Dow L Microb Biotechnol. 2025; 18(3):e70082.

PMID: 40040294 PMC: 11880119. DOI: 10.1111/1751-7915.70082.

Investigating the action model of the resistance enhancement induced by bacterial volatile organic compounds against in tomato fruit.

Chen J, Cao K, Lu X, Huang D, Ming R, Lu R Front Plant Sci. 2024; 15:1475416.

PMID: 39659409 PMC: 11628293. DOI: 10.3389/fpls.2024.1475416.

Volatile Organic Compounds Produced by Co-Culture of B418 with T11-W Exhibits Improved Antagonistic Activities against Fungal Phytopathogens.

Li W, Wang X, Jiang Y, Cui S, Hu J, Wei Y Int J Mol Sci. 2024; 25(20).

PMID: 39456879 PMC: 11507488. DOI: 10.3390/ijms252011097.

Insights on mining the pangenome of NMS02 S296 from the resistant banana cultivar confirms the antifungal action against f. sp. .

Ajesh B, Sariga R, Nakkeeran S, Renukadevi P, Saranya N, Alkahtani S Front Microbiol. 2024; 15:1443195.

PMID: 39364168 PMC: 11446778. DOI: 10.3389/fmicb.2024.1443195.

The Wheat Endophyte J4-3 Inhibits and Enhances Plant Growth.

Nzabanita C, Zhang L, Wang Y, Wang S, Guo L J Fungi (Basel). 2024; 10(1).

PMID: 38248920 PMC: 10817605. DOI: 10.3390/jof10010010.

References

Dolch M, Hornuss C, Klocke C, Praun S, Villinger J, Denzer W . Volatile organic compound analysis by ion molecule reaction mass spectrometry for Gram-positive bacteria differentiation. Eur J Clin Microbiol Infect Dis. 2012; 31(11):3007-13. DOI: 10.1007/s10096-012-1654-2. View

Carrion V, Cordovez V, Tyc O, Etalo D, de Bruijn I, de Jager V . Involvement of Burkholderiaceae and sulfurous volatiles in disease-suppressive soils. ISME J. 2018; 12(9):2307-2321. PMC: 6092406. DOI: 10.1038/s41396-018-0186-x. View

Zhang Y, Li T, Liu Y, Li X, Zhang C, Feng Z . Volatile Organic Compounds Produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 as Biological Fumigants To Control Ceratocystis fimbriata in Postharvest Sweet Potatoes. J Agric Food Chem. 2019; 67(13):3702-3710. DOI: 10.1021/acs.jafc.9b00289. View

Mahenthiralingam E, Bischof J, Byrne S, Radomski C, Davies J, Av-Gay Y . DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol. 2000; 38(9):3165-73. PMC: 87345. DOI: 10.1128/JCM.38.9.3165-3173.2000. View

Tyc O, Song C, Dickschat J, Vos M, Garbeva P . The Ecological Role of Volatile and Soluble Secondary Metabolites Produced by Soil Bacteria. Trends Microbiol. 2017; 25(4):280-292. DOI: 10.1016/j.tim.2016.12.002. View

Alijani Z, Amini J, Ashengroph M, Bahramnejad B . Antifungal activity of volatile compounds produced by Staphylococcus sciuri strain MarR44 and its potential for the biocontrol of Colletotrichum nymphaeae, causal agent strawberry anthracnose. Int J Food Microbiol. 2019; 307:108276. DOI: 10.1016/j.ijfoodmicro.2019.108276. View

Raza W, Ling N, Yang L, Huang Q, Shen Q . Response of tomato wilt pathogen Ralstonia solanacearum to the volatile organic compounds produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9. Sci Rep. 2016; 6:24856. PMC: 4840334. DOI: 10.1038/srep24856. View

Zhang Z, Li Y, Qi L, Wan X . Antifungal activities of major tea leaf volatile constituents toward Colletorichum camelliae Massea. J Agric Food Chem. 2006; 54(11):3936-40. DOI: 10.1021/jf060017m. View

Morya R, Salvachua D, Thakur I . Burkholderia: An Untapped but Promising Bacterial Genus for the Conversion of Aromatic Compounds. Trends Biotechnol. 2020; 38(9):963-975. DOI: 10.1016/j.tibtech.2020.02.008. View

10.

Mishra A, Singh S, Mahfooz S, Singh S, Bhattacharya A, Mishra N . Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress. Appl Environ Microbiol. 2018; 84(8). PMC: 5881058. DOI: 10.1128/AEM.02845-17. View

11.

Kai M, Haustein M, Molina F, Petri A, Scholz B, Piechulla B . Bacterial volatiles and their action potential. Appl Microbiol Biotechnol. 2008; 81(6):1001-12. DOI: 10.1007/s00253-008-1760-3. View

12.

Chambers A, Evans S, Folta K . Methyl anthranilate and γ-decalactone inhibit strawberry pathogen growth and achene Germination. J Agric Food Chem. 2013; 61(51):12625-33. DOI: 10.1021/jf404255a. View

13.

Tait E, Perry J, Stanforth S, Dean J . Identification of volatile organic compounds produced by bacteria using HS-SPME-GC-MS. J Chromatogr Sci. 2013; 52(4):363-73. DOI: 10.1093/chromsci/bmt042. View

14.

You M, Fang S, MacDonald J, Xu J, Yuan Z . Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth. Microbiol Res. 2019; 233:126395. DOI: 10.1016/j.micres.2019.126395. View

15.

Rathna J, Bakkiyaraj D, Pandian S . Anti-biofilm mechanisms of 3,5-di-tert-butylphenol against clinically relevant fungal pathogens. Biofouling. 2016; 32(9):979-93. DOI: 10.1080/08927014.2016.1216103. View

16.

Kong W, Li P, Wu X, Wu T, Sun X . Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi. Microorganisms. 2020; 8(4). PMC: 7232321. DOI: 10.3390/microorganisms8040590. View

17.

Wiesel L, Newton A, Elliott I, Booty D, Gilroy E, Birch P . Molecular effects of resistance elicitors from biological origin and their potential for crop protection. Front Plant Sci. 2014; 5:655. PMC: 4240061. DOI: 10.3389/fpls.2014.00655. View

18.

Coenye T, Vandamme P . Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol. 2003; 5(9):719-29. DOI: 10.1046/j.1462-2920.2003.00471.x. View

19.

Zhang D, Yu S, Yang Y, Zhang J, Zhao D, Pan Y . Antifungal Effects of Volatiles Produced by Against in Potato. Front Microbiol. 2020; 11:1196. PMC: 7311636. DOI: 10.3389/fmicb.2020.01196. View

20.

Jang Y, Jung J, Lee I, Kang S, Yun B . Nonanoic Acid, an Antifungal Compound from Hibiscus syriacus Ggoma. Mycobiology. 2012; 40(2):145-6. PMC: 3408307. DOI: 10.5941/MYCO.2012.40.2.145. View