Dynamic Changes in Aroma Compounds During Processing of Flat Black Tea: Combined GC-MS with Proteomic Analysis

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Oct 26

PMID 39456305

Authors

Cun Ao

Xiaojun Niu

Daliang Shi

Xuxia Zheng

Jizhong Yu

Yingbin Zhang

Affiliations

Soon will be listed here.

Abstract

Flat black tea (FBT) has been innovatively developed to alleviate homogenisation competition, but the dynamic changes in aroma components during the process remain unclear. This study employed HS-SPME-GC-MS to analyse the aroma components of tea samples from various processing stages of FBT, and to make a comparative assessment with conventional strip-like Congou black tea (SBT). Additionally, a proteomic analysis was conducted on fresh leaves, withered leaves, and frozen-thawed leaves. Significant changes were observed in the aroma components and proteins during the processing. The results of the multivariate and odour activity value analysis demonstrated that the principal aroma components present during the processing of FBT were linalool, (E)-2-hexen-1-al, methyl salicylate, geraniol, hexanal, benzeneacetaldehyde, (Z)-3-hexenyl butyrate, dimethyl sulphide, 2-methylbutanal, 2-ethylfuran, nonanal, nonanol, 3-methylbutanal, (Z)-3-hexen-1-ol, 2-pentylfuran, linalool oxide I, and β-myrcene. Freezing-thawing and final roasting are the key processing steps for forming the aroma quality of FBT. The final roasting yielded a considerable quantity of pyrazines and pyrroles, resulting in a high-fried aroma, but caused a significant reduction in linalool, geraniol, β-myrcene, and esters, which led to a loss of floral and fruity aromas. The freezing-thawing treatment resulted in an accelerated loss of aroma substances, accompanied by a decrease in the expression level of lipoxygenase and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. The formation of aroma substances in the linoleic acid metabolic pathway and terpenoid metabolic process was hindered, which had a negative impact on tea aroma. This study elucidates the causes of unsatisfactory aroma quality in tea products made from frozen tea leaves, providing theoretical support for the utilisation of frostbitten tea leaves, and helps us to understand the mechanism of aroma formation in black tea.

References

Chen Q, Zhu Y, Dai W, Lv H, Mu B, Li P . Aroma formation and dynamic changes during white tea processing. Food Chem. 2018; 274:915-924. DOI: 10.1016/j.foodchem.2018.09.072. View

Liao X, Yan J, Wang B, Meng Q, Zhang L, Tong H . Identification of key odorants responsible for cooked corn-like aroma of green teas made by tea cultivar 'Zhonghuang 1'. Food Res Int. 2020; 136:109355. DOI: 10.1016/j.foodres.2020.109355. View

Wu Q, Zhou Z, Zhang Y, Huang H, Ou X, Sun Y . Identification of Key Components Responsible for the Aromatic Quality of Jinmudan Black Tea by Means of Molecular Sensory Science. Foods. 2023; 12(9). PMC: 10178690. DOI: 10.3390/foods12091794. View

Chen S, Liu H, Zhao X, Li X, Shan W, Wang X . Non-targeted metabolomics analysis reveals dynamic changes of volatile and non-volatile metabolites during oolong tea manufacture. Food Res Int. 2020; 128:108778. DOI: 10.1016/j.foodres.2019.108778. View

Zhai X, Zhang L, Granvogl M, Ho C, Wan X . Flavor of tea (Camellia sinensis): A review on odorants and analytical techniques. Compr Rev Food Sci Food Saf. 2022; 21(5):3867-3909. DOI: 10.1111/1541-4337.12999. View

Zhu J, Chen F, Wang L, Niu Y, Xiao Z . Evaluation of the synergism among volatile compounds in Oolong tea infusion by odour threshold with sensory analysis and E-nose. Food Chem. 2016; 221:1484-1490. DOI: 10.1016/j.foodchem.2016.11.002. View

Yang Y, Hua J, Deng Y, Jiang Y, Qian M, Wang J . Aroma dynamic characteristics during the process of variable-temperature final firing of Congou black tea by electronic nose and comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. Food Res Int. 2020; 137:109656. DOI: 10.1016/j.foodres.2020.109656. View

Wu S, Liu X, Cheng L, Wang D, Qin G, Zhang X . Protective Mechanism of Leucine and Isoleucine against HO-Induced Oxidative Damage in Bovine Mammary Epithelial Cells. Oxid Med Cell Longev. 2022; 2022:4013575. PMC: 8964234. DOI: 10.1155/2022/4013575. View

Chen X, Chen D, Jiang H, Sun H, Zhang C, Zhao H . Aroma characterization of Hanzhong black tea (Camellia sinensis) using solid phase extraction coupled with gas chromatography-mass spectrometry and olfactometry and sensory analysis. Food Chem. 2018; 274():130-136. DOI: 10.1016/j.foodchem.2018.08.124. View

10.

Niu Y, Deng J, Xiao Z, Zhu J . Characterization of the major aroma-active compounds in peach (Prunus persica L. Batsch) by gas chromatography-olfactometry, flame photometric detection and molecular sensory science approaches. Food Res Int. 2021; 147:110457. DOI: 10.1016/j.foodres.2021.110457. View

11.

Fang X, Xu W, Jiang G, Sui M, Xiao J, Ning Y . Monitoring the dynamic changes in aroma during the whole processing of Qingzhuan tea at an industrial scale: From fresh leaves to finished tea. Food Chem. 2023; 439:137810. DOI: 10.1016/j.foodchem.2023.137810. View

12.

Dudareva N, Klempien A, Muhlemann J, Kaplan I . Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytol. 2013; 198(1):16-32. DOI: 10.1111/nph.12145. View

13.

Liu P, Zheng P, Feng L, Gong Z, Zheng L, Gao S . Dynamic changes in the aroma profile of Qingzhuan tea during its manufacture. Food Chem. 2021; 375:131847. DOI: 10.1016/j.foodchem.2021.131847. View

14.

Yang Y, Qian M, Deng Y, Yuan H, Jiang Y . Insight into aroma dynamic changes during the whole manufacturing process of chestnut-like aroma green tea by combining GC-E-Nose, GC-IMS, and GC × GC-TOFMS. Food Chem. 2022; 387:132813. DOI: 10.1016/j.foodchem.2022.132813. View

15.

Su D, He J, Zhou Y, Li Y, Zhou H . Aroma effects of key volatile compounds in Keemun black tea at different grades: HS-SPME-GC-MS, sensory evaluation, and chemometrics. Food Chem. 2021; 373(Pt B):131587. DOI: 10.1016/j.foodchem.2021.131587. View

16.

Wang Q, Xie J, Wang L, Jiang Y, Deng Y, Zhu J . Comprehensive investigation on the dynamic changes of volatile metabolites in fresh scent green tea during processing by GC-E-Nose, GC-MS, and GC × GC-TOFMS. Food Res Int. 2024; 187:114330. DOI: 10.1016/j.foodres.2024.114330. View

17.

Joshi R, Gulati A . Fractionation and identification of minor and aroma-active constituents in Kangra orthodox black tea. Food Chem. 2014; 167:290-8. DOI: 10.1016/j.foodchem.2014.06.112. View

18.

Wang H, Hua J, Yu Q, Li J, Wang J, Deng Y . Widely targeted metabolomic analysis reveals dynamic changes in non-volatile and volatile metabolites during green tea processing. Food Chem. 2021; 363:130131. DOI: 10.1016/j.foodchem.2021.130131. View

19.

Teng R, Ao C, Huang H, Shi D, Mao Y, Zheng X . Research of Processing Technology of Longjing Tea with 'Baiye 1' Based on Non-Targeted Aroma Metabolomics. Foods. 2024; 13(9). PMC: 11083364. DOI: 10.3390/foods13091338. View

20.

Zhu J, Zhu Y, Wang K, Niu Y, Xiao Z . Characterization of key aroma compounds and enantiomer distribution in Longjing tea. Food Chem. 2021; 361:130096. DOI: 10.1016/j.foodchem.2021.130096. View