Mutagenesis of Conserved 5' Elements and Transcription of a Chicken H1 Histone Gene

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Jan 24

PMID 3945554

Citations 8

Authors

H B Younghusband

R Sturm

J R Wells

Affiliations

Soon will be listed here.

Abstract

In addition to readily identifiable TATA and CAAT boxes, chicken H1 histone genes contain a highly conserved G-rich region at about minus 80 and an A-rich H1 histone gene-specific motif some 120 bases upstream from the H1 mRNA cap site. The level of transcription from wild-type, 'G-box' and 'A-box' H1 deletion mutant templates was tested in Xenopus oocytes and in HeLa cells. Removal of the H1 gene-specific motif had no effect on H1 gene transcription in either assay system, whereas deletion of the G-rich sequence decreased H1 mRNA levels by about ten-fold in oocytes and in HeLa cells. At least in heterologous systems, the H1-gene specific A-rich region does not appear to influence the level of H1 gene transcription.

Citing Articles

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Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription.

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A histone H1 protein in sea urchins is encoded by a poly(A)+ mRNA.

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