Molecular Typing of Isolates Collected in Abidjan Hospitals (Côte D'Ivoire) Using the Multiple-Locus Variable Number of Tandem Repeats Method

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2024 Oct 25

PMID 39451606

Authors

Christiane Essoh

Yolande Hauck

Timothee Ouassa

Daouda Toure

Richmond Djatchi

Guillaume Yao Loukou

Simon-Pierre Assanvo NGuetta

Gilles Vergnaud

Christine Pourcel

Affiliations

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Abstract

can cause community-acquired infections affecting various body sites. The present retrospective study investigated the genetic diversity of 173 isolates (166 clinical, 7 environmental) of collected from clinical pathology laboratories in Abidjan, Côte d'Ivoire (2001-2011). Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) using 13 loci was applied to all isolates and compared to published MLVA data. The antibiotics status of the isolates was compiled when available and compared to published profiles. Among 95 isolates analyzed for their antibiotics status, 14 displayed concerning resistance profiles: five multidrug-resistant (MDR) and nine extensively drug-resistant (XDR). MLVA typing revealed a high genetic diversity (>130 genotypes), with many genotypes represented by a single strain. Notably, thirteen clusters (≥4 related isolates) were observed. Some clusters displayed close genetic relatedness to isolates from France, Korea, and well-studied strains (ST560, LES and PA14). Comparative analysis suggested the presence of international high-risk MDR clones (CC233, CC111) in Côte d'Ivoire. Importantly, MLVA clustering revealed a close relationship of CC235-MDR strains with a locally identified cluster (group 9). These findings support MLVA as a reliable and cost-effective tool for low-resource settings, allowing the selection of relevant strains for future whole genome sequence analyses. This approach can improve outbreak investigations and public health interventions aimed at curbing MDR transmission within hospitals and at the national level.

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