The Buccal Fat Pad: A Unique Human Anatomical Structure and Rich and Easily Accessible Source of Mesenchymal Stem Cells for Tissue Repair

Overview

Journal Bioengineering (Basel)

Date 2024 Oct 25

PMID 39451344

Authors

Gaia Favero

Cornelis J F van Noorden

Rita Rezzani

Affiliations

Soon will be listed here.

Abstract

Buccal fat pads are biconvex adipose tissue bags that are uniquely found on both sides of the human face along the anterior border of the masseter muscles. Buccal fat pads are important determinants of facial appearance, facilitating gliding movements of facial masticatory and mimetic muscles. Buccal fad pad flaps are used for the repair of oral defects and as a rich and easily accessible source of mesenchymal stem cells. Here, we introduce the buccal fat pad anatomy and morphology and report its functions and applications for oral reconstructive surgery and for harvesting mesenchymal stem cells for clinical use. Future frontiers of buccal fat pad research are discussed. It is concluded that many biological and molecular aspects still need to be elucidated for the optimal application of buccal fat pad tissue in regenerative medicine.

References

Gholami L, Afshar S, Arkian A, Saeidijam M, Hendi S, Mahmoudi R . NIR irradiation of human buccal fat pad adipose stem cells and its effect on TRP ion channels. Lasers Med Sci. 2022; 37(9):3681-3692. DOI: 10.1007/s10103-022-03652-7. View

Kahn J, SICK H, Laude M, KORITKE J . [Vascularization of the adipose body of the cheek]. Arch Anat Histol Embryol. 1990; 73:3-20. View

Ghaderi H, Razmkhah M, Kiany F, Chenari N, Haghshenas M, Ghaderi A . Comparison of Osteogenic and Chondrogenic Differentiation Ability of Buccal Fat Pad Derived Mesenchymal Stem Cells and Gingival Derived Cells. J Dent (Shiraz). 2018; 19(2):124-131. PMC: 5960732. View

Han P, Johnson N, Abdal-Hay A, Moran C, Salomon C, Ivanovski S . Effects of periodontal cells-derived extracellular vesicles on mesenchymal stromal cell function. J Periodontal Res. 2023; 58(6):1188-1200. DOI: 10.1111/jre.13171. View

Homayouni A, Rezaie Rad M, Barikani H, Chiniforush N, Akbari S . Effect of 980 nm photobiomodulation delivered by a handpiece with Gaussian vs. Flat-Top profiles on proliferation and differentiation of buccal fat pad stem cells. Photochem Photobiol. 2024; 100(6):1902-1911. DOI: 10.1111/php.13929. View

OBrien J, Ashton M, Rozen W, Ross R, Mendelson B . New perspectives on the surgical anatomy and nomenclature of the temporal region: literature review and dissection study. Plast Reconstr Surg. 2012; 131(3):510-522. DOI: 10.1097/PRS.0b013e31827c6ed6. View

Arpornmaeklong P, Boonyuen S, Apinyauppatham K, Pripatnanont P . Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation. Bioengineering (Basel). 2024; 11(1). PMC: 10812978. DOI: 10.3390/bioengineering11010059. View

EGYEDI P . Utilization of the buccal fat pad for closure of oro-antral and/or oro-nasal communications. J Maxillofac Surg. 1977; 5(4):241-4. DOI: 10.1016/s0301-0503(77)80117-3. View

Lin M, Hazan E, John A, Dubin D, Younessi S, Khorasani H . Buccal Fat Pad Reduction With Intraoperative Fat Transfer to the Temple. Cutis. 2022; 109(1):46-48. DOI: 10.12788/cutis.0430. View

10.

Bereczki-Temistocle D, Gurzu S, Ioan I, Vlad G, Beresescu G, Ormenisan A . Successful Closure of an Oro-Antral Communication, after Three Previously Failed Attempts, Using Bichat Fat Pad Flap: A Case Report. World J Plast Surg. 2023; 12(1):90-94. PMC: 10200079. DOI: 10.52547/wjps.12.1.90. View

11.

van Noorden C, Breznik B, Novak M, van Dijck A, Tanan S, Vittori M . Cell Biology Meets Cell Metabolism: Energy Production Is Similar in Stem Cells and in Cancer Stem Cells in Brain and Bone Marrow. J Histochem Cytochem. 2021; 70(1):29-51. PMC: 8721571. DOI: 10.1369/00221554211054585. View

12.

Weissler J, Mohamed O, Gryskiewicz J, Chopra K . An Algorithmic Approach to Managing Parotid Duct Injury Following Buccal Fat Pad Removal. Aesthet Surg J Open Forum. 2022; 4:ojac032. PMC: 9154315. DOI: 10.1093/asjof/ojac032. View

13.

Rapidis A, Alexandridis C, Eleftheriadis E, Angelopoulos A . The use of the buccal fat pad for reconstruction of oral defects: review of the literature and report of 15 cases. J Oral Maxillofac Surg. 2000; 58(2):158-63. DOI: 10.1016/s0278-2391(00)90330-6. View

14.

Nelke K, Morawska A, Blaszczyk B, Janeczek M, Pasicka E, Lukaszewski M . Anatomical and Surgical Implications of the Usage of Bichat Fat Pad in Oroantral Communication, Maxillary, Palatal, and Related Surgeries-Narrative Review. J Clin Med. 2023; 12(15). PMC: 10419993. DOI: 10.3390/jcm12154909. View

15.

Dolanmaz D, Tuz H, Bayraktar S, Metin M, Erdem E, Baykul T . Use of pedicled buccal fat pad in the closure of oroantral communication: analysis of 75 cases. Quintessence Int. 2004; 35(3):241-6. View

16.

Ardeshirylajimi A, Mossahebi-Mohammadi M, Vakilian S, Langroudi L, Seyedjafari E, Atashi A . Comparison of osteogenic differentiation potential of human adult stem cells loaded on bioceramic-coated electrospun poly (L-lactide) nanofibres. Cell Prolif. 2014; 48(1):47-58. PMC: 6496866. DOI: 10.1111/cpr.12156. View

17.

Li Y, Mei Z, Li J, Gimble J . Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates. Methods Mol Biol. 2024; 2783:13-24. DOI: 10.1007/978-1-0716-3762-3_2. View

18.

Kim C, Takeshita Y, Kugimoto T, Harada H, Park J, Tubbs R . Anatomy of the Buccal Space: Surgical and Radiological Perspectives. J Craniofac Surg. 2024; 35(7):1972-1976. DOI: 10.1097/SCS.0000000000010411. View

19.

Broccaioli E, Niada S, Rasperini G, Ferreira L, Arrigoni E, Yenagi V . Mesenchymal Stem Cells from Bichat's Fat Pad: In Vitro Comparison with Adipose-Derived Stem Cells from Subcutaneous Tissue. Biores Open Access. 2013; 2(2):107-17. PMC: 3620540. DOI: 10.1089/biores.2012.0291. View

20.

Vieira G, Jorge F, Franco E, Dias L, Guimaraes M, Alves de Oliveira L . Lesions of the Parotid Gland and Buccal Artery After Buccal Fat Pad Reduction. J Craniofac Surg. 2018; 30(3):790-792. DOI: 10.1097/SCS.0000000000004880. View