NIR Irradiation of Human Buccal Fat Pad Adipose Stem Cells and Its Effect on TRP Ion Channels

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2022 Oct 13

PMID 36227520

Authors

Leila Gholami

Saeid Afshar

Aliasghar Arkian

Masood Saeidijam

Seyedeh Sareh Hendi

Roghayeh Mahmoudi

Khatereh Khorsandi

Hadi Hashemzehi

Reza Fekrazad

Affiliations

Soon will be listed here.

Abstract

The effect of near infrared (NIR) laser irradiation on proliferation and osteogenic differentiation of buccal fat pad-derived stem cells and the role of transient receptor potential (TRP) channels was investigated in the current research. After stem cell isolation, a 940 nm laser with 0.1 W, 3 J/cm was used in pulsed and continuous mode for irradiation in 3 sessions once every 48 h. The cells were cultured in the following groups: non-osteogenic differentiation medium/primary medium (PM) and osteogenic medium (OM) groups with laser-irradiated (L +), without irradiation (L -), laser treated + Capsazepine inhibitor (L + Cap), and laser treated + Skf96365 inhibitor (L + Skf). Alizarin Red staining and RT-PCR were used to assess osteogenic differentiation and evaluate RUNX2, Osterix, and ALP gene expression levels. The pulsed setting showed the best viability results (P < 0.05) and was used for osteogenic differentiation evaluations. The results of Alizarin red staining were not statistically different between the four groups. Osterix and ALP expression increased in the (L +) group. This upregulation abrogated in the presence of Capsazepine, TRPV1 inhibitor (L + Cap); however, no significant effect was observed with Skf96365 (L + Skf).

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