Protocol to Investigate G Protein-coupled Receptor Signaling Kinetics and Concentration-dependent Responses Using ONE-GO Biosensors

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Oct 12

PMID 39395175

Authors

Remi Janicot

Mikel Garcia-Marcos

Affiliations

Soon will be listed here.

Abstract

ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein families (G, G, G, and G) permits the study of virtually any GPCR. Here, we present a protocol to implement ONE-GO biosensors in cell lines to investigate GPCR signaling kinetics and concentration-dependent responses. We describe steps for cell culture and transfection, response measurement, and data analysis. For complete details on the use and execution of this protocol, please refer to Janicot et al..

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