Systematic Optimization of UCNPs-LFA for Helicobacter Pylori Nucleic Acid Detection at Point-of-care

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2024 Oct 6

PMID 39370436

Authors

Birui Jin

Siyu Li

Chuyao Zhang

Chuan Ma

Jie Hu

Jun Wang

Zedong Li

Affiliations

Soon will be listed here.

Abstract

Helicobacter pylori (Hp) prevail globally as the primary cause of gastritis, gastric ulcer, and potential gastric cancer, highlighting the need for rapid and precise point-of-care (POC) detection of Hp nucleic acid. Upconversion nanoparticle-based lateral flow assay (UCNPs-LFA) exhibit great potential in POC detection, due to their high optical stability and absence of background fluorescence. However, insufficient sensitivity for nucleic acid detection remains a key challenge. This study systematically optimizes UCNPs-LFA by focusing on target capture, signal transduction, signal separation, and signal analysis, to enhance its detection capabilities for Hp nucleic acid. The optimized UCNPs-LFA platform features a significantly decreased detection limit, a broadened detection range, and high reliability. Results demonstrate that the limit of detection (LOD) is 25 fM, a 10-fold improvement over the initial platform. This systematic optimization strategy is versatile and can be applied to optimize other nanoparticle-based LFAs.

References

Aziz F, Khan I, Shukla S, Dey D, Yan Q, Chakraborty A . Partners in crime: The Lewis Y antigen and fucosyltransferase IV in Helicobacter pylori-induced gastric cancer. Pharmacol Ther. 2021; 232:107994. DOI: 10.1016/j.pharmthera.2021.107994. View

Gholizadeh Navashenaq J, Shabgah A, Banach M, Jamialahmadi T, Penson P, Johnston T . The interaction of Helicobacter pylori with cancer immunomodulatory stromal cells: New insight into gastric cancer pathogenesis. Semin Cancer Biol. 2021; 86(Pt 3):951-959. DOI: 10.1016/j.semcancer.2021.09.014. View

Hu S, Zhang X, Li W, Chen L, Zhao W, Xu J . Identification of multiple single-nucleotide variants for clinical evaluation of Helicobacter pylori drug resistance. Talanta. 2022; 243:123367. DOI: 10.1016/j.talanta.2022.123367. View

Jiang P, Dong K, Zhang W, Wang H, Xiao X, Chen N . A universal probe system for low-abundance point mutation detection based on endonuclease IV. Analyst. 2022; 147(8):1534-1539. DOI: 10.1039/d1an02003j. View

Long S . In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the Raindance ddPCR platform. Methods. 2021; 201:82-95. PMC: 8501152. DOI: 10.1016/j.ymeth.2021.04.008. View

Blumenfeld N, Bolene M, Jaspan M, Ayers A, Zarrandikoetxea S, Freudman J . Multiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis. Nat Nanotechnol. 2022; 17(9):984-992. DOI: 10.1038/s41565-022-01175-4. View

Li X, Li G, Pan Q, Xue F, Wang Z, Peng C . Rapid and ultra-sensitive lateral flow assay for pathogens based on multivalent aptamer and magnetic nanozyme. Biosens Bioelectron. 2024; 250:116044. DOI: 10.1016/j.bios.2024.116044. View

Li G, Li Q, Wang X, Liu X, Zhang Y, Li R . Lateral flow immunoassays for antigens, antibodies and haptens detection. Int J Biol Macromol. 2023; 242(Pt 4):125186. PMC: 10232721. DOI: 10.1016/j.ijbiomac.2023.125186. View

Arai M, Kim H, Pascavis M, Cha B, Brambilla G, Cho Y . Upconverting Nanoparticle-based Enhanced Luminescence Lateral-Flow Assay for Urinary Biomarker Monitoring. ACS Appl Mater Interfaces. 2024; 16(29):38243-38251. DOI: 10.1021/acsami.4c06117. View

10.

Wang C, Xiao R, Wang S, Yang X, Bai Z, Li X . Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples. Biosens Bioelectron. 2019; 146:111754. DOI: 10.1016/j.bios.2019.111754. View

11.

Jin B, Du Z, Zhang C, Yu Z, Wang X, Hu J . Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection. Biosensors (Basel). 2022; 12(11). PMC: 9688000. DOI: 10.3390/bios12110977. View

12.

Roy S, Curry S, Bagot C, Mueller E, Mansouri A, Park W . Enzyme Prodrug Therapy with Photo-Cross-Linkable Anti-EGFR Affibodies Conjugated to Upconverting Nanoparticles. ACS Nano. 2022; 16(10):15873-15883. PMC: 10197967. DOI: 10.1021/acsnano.2c02558. View

13.

Zhang K, Zhao Q, Qin S, Fu Y, Liu R, Zhi J . Nanodiamonds conjugated upconversion nanoparticles for bio-imaging and drug delivery. J Colloid Interface Sci. 2018; 537:316-324. DOI: 10.1016/j.jcis.2018.11.028. View

14.

Hofmann A, Saft B, Reich P, Grabmann M, Glaser G, Trubenbach M . Lock-In Pixel CMOS Image Sensor for Time-Resolved Fluorescence Readout of Lateral-Flow Assays. IEEE Trans Biomed Circuits Syst. 2022; 16(4):535-544. DOI: 10.1109/TBCAS.2022.3192926. View

15.

Rimbara E, Noguchi N, Kawai T, Sasatsu M . Novel mutation in 23S rRNA that confers low-level resistance to clarithromycin in Helicobacter pylori. Antimicrob Agents Chemother. 2008; 52(9):3465-6. PMC: 2533477. DOI: 10.1128/AAC.00445-08. View

16.

Jin B, Du Z, Ji J, Bai Y, Tang D, Qiao L . Regulation of probe density on upconversion nanoparticles enabling high-performance lateral flow assays. Talanta. 2023; 256:124327. DOI: 10.1016/j.talanta.2023.124327. View

17.

Hu J, Wang L, Li F, Han Y, Lin M, Lu T . Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays. Lab Chip. 2013; 13(22):4352-7. DOI: 10.1039/c3lc50672j. View

18.

Tang R, Alam N, Li M, Xie M, Ni Y . Dissolvable sugar barriers to enhance the sensitivity of nitrocellulose membrane lateral flow assay for COVID-19 nucleic acid. Carbohydr Polym. 2021; 268:118259. DOI: 10.1016/j.carbpol.2021.118259. View

19.

Gong Y, Zheng Y, Jin B, You M, Wang J, Li X . A portable and universal upconversion nanoparticle-based lateral flow assay platform for point-of-care testing. Talanta. 2019; 201:126-133. DOI: 10.1016/j.talanta.2019.03.105. View

20.

Li T, Hu R, Xia J, Xu Z, Chen D, Xi J . G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection. Biosens Bioelectron. 2021; 187:113292. DOI: 10.1016/j.bios.2021.113292. View