Golden Gate Cloning for Efficient Biosynthesis of Lycopene in Synthetic Yeast

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Oct 3

PMID 39363085

Authors

Yu Zhang

Ju Zheng

Xian Fu

Yue Shen

Affiliations

Soon will be listed here.

Abstract

Golden Gate Assembly (GGA) represents a versatile method for assembling multiple DNA fragments into a single molecule, which is widely used in rapid construction of complex expression cassettes for metabolic engineering. Here we describe the GGA method for facile construction and optimization of lycopene biosynthesis pathway by the combinatorial assembly of different transcriptional units (TUs). Furthermore, we report the method for characterizing and improving lycopene production in the synthetic yeast chassis.

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