Effects of Multisuperovulation on the Transcription and Genomic Methylation of Oocytes and Offspring

Overview

Journal Clin Epigenetics

Publisher Biomed Central

Specialty Genetics

Date 2024 Sep 28

PMID 39342274

Authors

Juan-Ke Xie

Qian Wang

Yuan-Hui Chen

Shou-Bin Tang

Hao-Yue Sun

Zhao-Jia Ge

Cui-Lian Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Controlled ovarian stimulation is a common skill of assisted reproductive technologies (ARTs). In the clinic, some females would undergo more than one controlled ovarian stimulation cycle. However, few studies have focused on the influence of multi-superovulation on oocytes and offspring.

Results: Here, we found that multi-superovulation disrupted the transcriptome of oocytes and that the differentially expressed genes (DEGs) were associated mainly with metabolism and fertilization. The disruption of mRNA degradation via poly (A) size and metabolism might be a reason for the reduced oocyte maturation rate induced by repeated superovulation. Multi-superovulation results in hypo-genomic methylation in oocytes. However, there was an increase in the methylation level of CGIs. The DMRs are not randomly distributed in genome elements. Genes with differentially methylated regions (DMRs) in promoters are enriched in metabolic pathways. With increasing of superovulation cycles, the glucose and insulin tolerance of offspring is also disturbed.

Conclusions: These results suggest that multi-superovulation has adverse effects on oocyte quality and offspring health.

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