Spontaneous Induction of Colicin E1 in Escherichia Coli Strains Deficient in Both Exonucleases I and V

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Dec 1

PMID 3934144

Authors

C L Bassett

S R Kushner

Affiliations

Soon will be listed here.

Abstract

Colicin E1 synthesis is spontaneously induced in pRSF2124-carrying strains of Escherichia coli deficient in exonucleases I (sbcB) and V (recB recC). In contrast, the specific activity of beta-lactamase, which is also encoded by pRSF2124, is not affected by the absence of these enzymes. These results suggest that colicin E1 induction is specific and does not result either from a significant change in overall plasmid transcription or copy number. Furthermore, the level of spontaneous induction was similar to that obtained with mitomycin C.

References

Herschman H, Helinski D . Comparative study of the events associated with colicin induction. J Bacteriol. 1967; 94(3):691-9. PMC: 251940. DOI: 10.1128/jb.94.3.691-699.1967. View

Imsande J . NEW ASSAY FOR PENICILLINASE AND SOME RESULTS ON PENICILLINASE INDUCTION. J Bacteriol. 1965; 89:1322-7. PMC: 277647. DOI: 10.1128/jb.89.5.1322-1327.1965. View

Linstrom E, Boman H, STEELE B . Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains. J Bacteriol. 1970; 101(1):218-31. PMC: 250473. DOI: 10.1128/jb.101.1.218-231.1970. View

Kushner S, Nagaishi H, Templin A, Clark A . Genetic recombination in Escherichia coli: the role of exonuclease I. Proc Natl Acad Sci U S A. 1971; 68(4):824-7. PMC: 389052. DOI: 10.1073/pnas.68.4.824. View

Kennedy C . Induction of colicin production by high temperature or inhibition of protein synthesis. J Bacteriol. 1971; 108(1):10-9. PMC: 247026. DOI: 10.1128/jb.108.1.10-19.1971. View

Hardy K, Meynell G . Colicin factors and mitomycin-C. J Gen Microbiol. 1972; 73(3):547-9. DOI: 10.1099/00221287-73-3-547. View

Hull R . Effect of tsl mutations on Col E1 expression in a recA strain of Escherichia coli K-12. J Bacteriol. 1975; 123(2):775-6. PMC: 235792. DOI: 10.1128/jb.123.2.775-776.1975. View

So M, Gill R, Falkow S . The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet. 1975; 142(3):239-49. DOI: 10.1007/BF00425649. View

Gould J, Cramer W . Studies on the depolarization of the Escherichia coli cell membrane by colicin E1. J Biol Chem. 1977; 252(15):5491-7. View

10.

. Colicins and bacterial membranes: structures and functions. I. Effects of colicins on the protein composition of the membranes of sensitive and tolerant Escherichia coli. J Biol Chem. 1978; 253(3):982-9. View

11.

Uhlin B, Nordstrom K . R plasmid gene dosage effects in Escherichia coli K-12: copy mutants of the R plasmic R1drd-19. Plasmid. 1977; 1(1):1-7. DOI: 10.1016/0147-619x(77)90003-8. View

12.

Ohkubo H, Shimada K, Takagi Y . Isolation and characterization of a mutant ColE1 plasmid that allows constitutive colicin E1 synthesis. J Bacteriol. 1979; 139(1):311-5. PMC: 216862. DOI: 10.1128/jb.139.1.311-315.1979. View

13.

Shafferman A, Flashner Y, Cohen S . ColE1 DNA sequences interacting in cis, essential for mitomycin-C induced lethality. Mol Gen Genet. 1979; 176(1):139-46. DOI: 10.1007/BF00334305. View

14.

TESSMAN E, Peterson P . tif-dependent induction of colicin E1, prophage lambda, and filamentation in Escherichia coli K-12. J Bacteriol. 1980; 143(3):1307-17. PMC: 294502. DOI: 10.1128/jb.143.3.1307-1317.1980. View

15.

Little J, Edmiston S, Pacelli L, Mount D . Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci U S A. 1980; 77(6):3225-9. PMC: 349587. DOI: 10.1073/pnas.77.6.3225. View

16.

Ebina Y, Kishi F, Miki T, KAGAMIYAMA H, Nakazawa T, Nakazawa A . The nucleotide sequence surrounding the promoter region of colicin E1 gene. Gene. 1981; 15(2-3):119-26. DOI: 10.1016/0378-1119(81)90121-9. View

17.

Ebina Y, Kishi F, Nakazawa A . Direct participation of lexA protein in repression of colicin E1 synthesis. J Bacteriol. 1982; 150(3):1479-81. PMC: 216377. DOI: 10.1128/jb.150.3.1479-1481.1982. View

18.

Karu A, Belk E . Induction of E. coli recA protein via recBC and alternate pathways: quantitation by enzyme-linked immunosorbent assay (ELISA). Mol Gen Genet. 1982; 185(2):275-82. DOI: 10.1007/BF00330798. View

19.

Sabik J, Suit J, Luria S . cea-kil operon of the ColE1 plasmid. J Bacteriol. 1983; 153(3):1479-85. PMC: 221799. DOI: 10.1128/jb.153.3.1479-1485.1983. View

20.

Bachmann B . Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983; 47(2):180-230. PMC: 281571. DOI: 10.1128/mr.47.2.180-230.1983. View