Non-Ionic Fluorosurfactants for Droplet-Based in Vivo Applications

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2024 Sep 28

PMID 39340199

Authors

Heidi L van de Wouw

Shuo-Ting Yen

Manon Valet

Joseph A Garcia

Carlos O Gomez

Antoine Vian

Yucen Liu

Jennifer Pollock

Petr Pospisil

Otger Campas

Ellen M Sletten

Affiliations

Soon will be listed here.

Abstract

Fluorocarbon oils are uniquely suited for many biomedical applications due to their inert, bioorthogonal properties. In order to interface fluorocarbon oils with biological systems, non-ionic fluorosurfactants are necessary. However, there is a paucity of non-ionic fluorosurfactants with low interfacial tension (IFT) to stabilize fluorocarbon phases in aqueous environments (such as oil-in-water emulsions). We developed non-ionic fluorosurfactants composed of a polyethylene glycol (PEG) segment covalently bonded to a flexible perfluoropolyether (PFPE) segment that confer low IFTs between a fluorocarbon oil (HFE-7700) and water. The synthesis of a panel of surfactants spanning a molecular weight range of 0.64-66 kDa with various hydrophilic-lipophilic balances allowed for identification of minimal IFTs, ranging from 1.4 to 17.8 mN m. The majority of these custom fluorosurfactants display poor solubility in water, allowing their co-introduction with fluorocarbon oils and minimal leaching. We applied the PEGPFPE surfactant for mechanical force measurements in zebrafish, enabling exceptional sensitivity.

Citing Articles

Non-Ionic Fluorosurfactants for Droplet-Based in vivo Applications.

van de Wouw H, Yen S, Valet M, Garcia J, Gomez C, Vian A Angew Chem Int Ed Engl. 2024; 63(52):e202404956.

PMID: 39340199 PMC: 11656150. DOI: 10.1002/anie.202404956.

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