Antibacterial Activity and Prebiotic Properties of Six Types of Lamiaceae Honey

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2024 Sep 28

PMID 39335041

Authors

Filomena Nazzaro

Maria Neve Ombra

Francesca Coppola

Beatrice De Giulio

Antonio dAcierno

Raffaele Coppola

Florinda Fratianni

Affiliations

Soon will be listed here.

Abstract

Our work investigated the antimicrobial and prebiotic properties of basil, mint, oregano, rosemary, savory, and thyme honey. The potential antimicrobial action, assessed against the pathogens , , , , and , evidenced the capacity of the honey to influence the pathogenic hydrophobicity and hemolytic activities. Honey inhibited pathogen biofilms, acting especially on the mature biofilms, with inhibition rates of up to 81.62% (caused by the presence of mint honey on ). biofilms were the most susceptible to the presence of honey, with inhibition rates up of to 67.38% in the immature form (caused by basil honey) and up to 80.32% in the mature form (caused by mint honey). In some cases, the amount of nuclear and proteic material, evaluated by spectrophotometric readings, if also related to the honey's biofilm inhibitory activity, let us hypothesize a defective capacity of building the biofilm scaffold or bacterial membrane damage or an incapability of producing them for the biofilm scaffold. The prebiotic potentiality of the honey was assessed on Shirota, , subsp. , and and indicated their capacity to affect the whole probiotic growth and in vitro adhesive capacity, as well as the antioxidant and cytotoxic abilities, and to inhibit, mainly in the test performed with the Shirota, , and supernatants, the immature biofilm of the pathogens mentioned above.

References

Ijaz Hussain A, Anwar F, Sherazi S, Przybylski R . Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2015; 108(3):986-95. DOI: 10.1016/j.foodchem.2007.12.010. View

Schell K, Fernandes K, Shanahan E, Wilson I, Blair S, Carter D . The Potential of Honey as a Prebiotic Food to Re-engineer the Gut Microbiome Toward a Healthy State. Front Nutr. 2022; 9:957932. PMC: 9367972. DOI: 10.3389/fnut.2022.957932. View

Qanash H, Bazaid A, Binsaleh N, Patel M, Althomali O, Bin Sheeha B . In Vitro Antiproliferative Apoptosis Induction and Cell Cycle Arrest Potential of Saudi Sidr Honey against Colorectal Cancer. Nutrients. 2023; 15(15). PMC: 10421499. DOI: 10.3390/nu15153448. View

Masad R, Idriss I, Mohamed Y, Al-Sbiei A, Bashir G, Al-Marzooq F . Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition. Front Immunol. 2024; 15:1354297. PMC: 10912506. DOI: 10.3389/fimmu.2024.1354297. View

Merckoll P, Jonassen T, Vad M, Jeansson S, Melby K . Bacteria, biofilm and honey: a study of the effects of honey on 'planktonic' and biofilm-embedded chronic wound bacteria. Scand J Infect Dis. 2009; 41(5):341-7. DOI: 10.1080/00365540902849383. View

Wan Y, Wang X, Bai T, Zheng X, Yang L, Li Q . Lysine Inhibits Hemolytic Activity of and Its Application in Food Model Contaminated with . Toxins (Basel). 2022; 14(12). PMC: 9786064. DOI: 10.3390/toxins14120867. View

Shen L, Liu D, Li M, Jin F, Din M, Parnell L . Mechanism of action of recombinant acc-royalisin from royal jelly of Asian honeybee against gram-positive bacteria. PLoS One. 2012; 7(10):e47194. PMC: 3467205. DOI: 10.1371/journal.pone.0047194. View

Afrin S, Giampieri F, Gasparrini M, Forbes-Hernandez T, Cianciosi D, Reboredo-Rodriguez P . The inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cell growth. Part 1: the suppression of cell proliferation, promotion of apoptosis and arrest of the cell cycle. Food Funct. 2018; 9(4):2145-2157. DOI: 10.1039/c8fo00164b. View

Berne C, Ellison C, Ducret A, Brun Y . Bacterial adhesion at the single-cell level. Nat Rev Microbiol. 2018; 16(10):616-627. DOI: 10.1038/s41579-018-0057-5. View

10.

Maikranz E, Spengler C, Thewes N, Thewes A, Nolle F, Jung P . Different binding mechanisms of Staphylococcus aureus to hydrophobic and hydrophilic surfaces. Nanoscale. 2020; 12(37):19267-19275. DOI: 10.1039/d0nr03134h. View

11.

Wang Z, Elimelech M, Lin S . Environmental Applications of Interfacial Materials with Special Wettability. Environ Sci Technol. 2016; 50(5):2132-50. DOI: 10.1021/acs.est.5b04351. View

12.

Sarker S, Nahar L, Kumarasamy Y . Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007; 42(4):321-4. PMC: 1895922. DOI: 10.1016/j.ymeth.2007.01.006. View

13.

Nishio E, Ribeiro J, Oliveira A, Andrade C, Proni E, Kobayashi R . Antibacterial synergic effect of honey from two stingless bees: Scaptotrigona bipunctata Lepeletier, 1836, and S. postica Latreille, 1807. Sci Rep. 2016; 6:21641. PMC: 4751499. DOI: 10.1038/srep21641. View

14.

Alzahrani H, Alsabehi R, Boukraa L, Abdellah F, Bellik Y, Bakhotmah B . Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins. Molecules. 2012; 17(9):10540-9. PMC: 6268398. DOI: 10.3390/molecules170910540. View

15.

Jia Y, Guan Z, Liu C, Huang M, Li J, Feng J . β-hemolysin causes skin inflammation by acting as an agonist of epidermal growth factor receptor. Microbiol Spectr. 2023; 12(1):e0222723. PMC: 10783061. DOI: 10.1128/spectrum.02227-23. View

16.

Fratianni F, De Giulio B, dAcierno A, Amato G, De Feo V, Coppola R . In Vitro Prebiotic Effects and Antibacterial Activity of Five Leguminous Honeys. Foods. 2023; 12(18). PMC: 10529961. DOI: 10.3390/foods12183338. View

17.

Jacobsen C, Rosenfeldt Nielsen V, Hayford A, Moller P, Michaelsen K, Paerregaard A . Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl Environ Microbiol. 1999; 65(11):4949-56. PMC: 91666. DOI: 10.1128/AEM.65.11.4949-4956.1999. View

18.

Rajoka M, Zhao H, Lu Y, Lian Z, Li N, Hussain N . Anticancer potential against cervix cancer (HeLa) cell line of probiotic Lactobacillus casei and Lactobacillus paracasei strains isolated from human breast milk. Food Funct. 2018; 9(5):2705-2715. DOI: 10.1039/c8fo00547h. View

19.

Kullisaar T, Zilmer M, Mikelsaar M, Vihalemm T, Annuk H, Kairane C . Two antioxidative lactobacilli strains as promising probiotics. Int J Food Microbiol. 2002; 72(3):215-24. DOI: 10.1016/s0168-1605(01)00674-2. View

20.

Cane L, Saffioti N, Genetet S, Daza Millone M, Ostuni M, Schwarzbaum P . Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins. Biochimie. 2023; 216:3-13. DOI: 10.1016/j.biochi.2023.10.008. View