Ex Vivo Simultaneous HO Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys-A Feasibility Study

Overview

Journal J Imaging

Publisher MDPI

Specialty Radiology

Date 2024 Sep 27

PMID 39330429

Authors

Maibritt Meldgaard Arildsen

Christian Ostergaard Mariager

Christoffer Vase Overgaard

Thomas Vorre

Martin Bojesen

Niels Moeslund

Aage Kristian Olsen Alstrup

Lars Poulsen Tolbod

Mikkel Holm Vendelbo

Steffen Ringgaard

Michael Pedersen

Niels Henrik Buus

Affiliations

Soon will be listed here.

Abstract

Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a HO PET sequence for determination of RBP.

Results: RBP was measured using HO PET in all kidneys (flow 1: 0.42-0.76 mL/min/g, flow 2: 0.7-1.6 mL/min/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r = 0.87).

Conclusion: Our study demonstrated the feasibility of combined HO PET/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.

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