Enhancing Dental Alginate with , and Green Silver Nanoparticles: A Nature-Enhanced Approach for Superior Infection Control

Overview

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Date 2024 Sep 27

PMID 39330202

Authors

Lamia Singer

Leonie Beuter

Sabina Karacic

Gabriele Bierbaum

Jesenko Karacic

Christoph Bourauel

Affiliations

Soon will be listed here.

Abstract

Objectives: The study addresses the challenge of cross-infection in dentistry, focusing on improving disinfection protocols for dental hydrocolloid gel materials. This research aimed to incorporate chlorhexidine, natural plant extracts, and green-synthesized silver nanoparticles (AgNPs) into dental alginate to enhance safety and hygiene standards in dental practices.

Methods: Conventional dental alginate served as the control, with experimental groups including alginate modified with 0.2% chlorhexidine (CHX-group) and alginate incorporating AgNPs synthesized from () and () extracts (SA + AgNPs and ZO + AgNPs groups). Plant extracts were analyzed via GC/MS to confirm their composition. UV-visible spectroscopy, SEM, and EDX characterized the synthesized AgNPs. Antimicrobial efficacy against , and methicillin-resistant and methicillin-sensitive was evaluated using agar well diffusion assays. The dimensional accuracy of alginate impressions was assessed according to ISO 21563:2021 standards.

Results: Chemical analysis of and extracts identified 60 and 43 active compounds, respectively, supporting their use in AgNP synthesis. UV-visible spectroscopy, SEM, and EDX confirmed the formation of spherical AgNPs using and extracts. Modified groups showed inhibitory activity against and methicillin-resistant (MRSA) and methicillin-sensitive (MSSA), in contrast to the unmodified control. Both AgNP-modified groups demonstrated efficacy comparable to the CHX-group against MRSA and MSSA, with SA + AgNP showing superior performance against . The dimensional accuracy of all groups was within clinically acceptable ranges as reported in the literature (0.027-0.083 mm).

Discussion: CHX, silver nitrate, and green-synthesized AgNPs present promising options for developing self-disinfecting alginate impression materials. Utilizing plant extracts in AgNP synthesis offers a safe, efficient, and synergistic approach between metal ions and phytotherapeutic agents. This approach could potentially enhance disinfection efficacy without compromising material performance, improving dental safety and hygiene.

Citing Articles

Assessing the impact of an environmentally friendly approach on irreversible dental hydrocolloid performance.

Beuter L, Bourauel C, Singer L Sci Rep. 2024; 14(1):30516.

PMID: 39681606 PMC: 11649782. DOI: 10.1038/s41598-024-83035-w.

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