Effect of Advanced Maternal Age on Ischemic Stroke Vulnerability in Aged Rats: Investigating on Blood-brain Barrier Permeability and Gene Expression

Overview

Journal Aging Brain

Publisher Elsevier

Specialty Geriatrics

Date 2024 Sep 23

PMID 39309404

Authors

Samira Khayat

Hamed Fanaei

Affiliations

Soon will be listed here.

Abstract

Background: Advanced maternal age (AMA), commonly defined as pregnancy at or above 35 years old. Based on the evidence, this trend has raised concerns about potential health consequences for mothers, particularly in relation to ischemic stroke. Studies suggest that AMA may be associated with a higher risk of ischemic stroke in women due to physiological changes that impact vascular health and increase cardiovascular risk factors. The aim of this study was to investigate the effect of AMA on the extent of damage after ischemic stroke in aged rats.

Methods: Female rats that gave birth at an old age (10 months) and at a young age (4 months) were subjected to ischemic stroke in old age (20 months) and subsequently compared.We assessed neurological deficits, infarct volume, blood-brain barrier (BBB) permeability, TNF-alpha levels, total oxidant capacity, and gene expressions that play a role in BBB integrity (VEGF, Occludin, and MMP-9) following ischemic stroke.

Results: There were significantly elevated levels of MMP-9 expression and reduced levels of occludin in AMA rats. Additionally, AMA rats had significantly higher levels of TNF-alpha and total oxidant capacity after experiencing an ischemic stroke. AMA rats showed significantly higher brain water content (BBB permeability), infarct volume, and neurological deficits compared to young-aged pregnancies.

Discussion: Complex relationship between pregnancy-related physiological changes, aging, vascular gene expression, and inflammatory factors may play a role in the increased vulnerability observed in older pregnant rats. The similarities between pregnancy-related alterations and aging highlight the influence of advanced maternal age on susceptibility to ischemic stroke.

Citing Articles

Aging, vascular dysfunction, and the blood-brain barrier: unveiling the pathophysiology of stroke in older adults.

Alaqel S, Imran M, Khan A, Nayeem N Biogerontology. 2025; 26(2):67.

PMID: 40044939 DOI: 10.1007/s10522-025-10209-y.

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