Identification and Functional Exploration of Genes Reveal Their Potential Roles in Drought Stress Tolerance and Sexual Reproduction in L. Ssp.

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Sep 14

PMID 39273590

Authors

Yanting Zhao

Xinjie Sun

Jingyuan Zhou

Lixuan Liu

Li Huang

Qizan Hu

Affiliations

Soon will be listed here.

Abstract

Gibberellic acid-stimulated Arabidopsis sequences () are a subset of the gibberellin (GA)-regulated gene family and play crucial roles in various physiological processes. However, the genes in have not yet been documented. In this study, we identified and characterized 16 genes in Chinese cabbage ( L. ssp. ). Analysis of the conserved motifs revealed significant conservation within the activation segment of genes. This gene family contains numerous promoter elements associated with abiotic stress tolerance, including those for abscisic acid (ABA) and methyl jasmonate (MeJA). Expression profiling revealed the presence of these genes in various tissues, including roots, stems, leaves, flowers, siliques, and callus tissues. When plants were exposed to drought stress, the expression of decreased notably in drought-sensitive genotypes compared to their wild-type counterparts, highlighting the potentially crucial role of in drought stress. Additionally, exhibited various functions in sexual reproduction dynamics. The findings contribute to the understanding of the function of and provide valuable insights for further exploration of the gene function of the gene in Chinese cabbage.

Citing Articles

Genome-Wide Analysis of BURP Domain-Containing Gene Family in and Functional Analysis of Under Drought and Salt Stresses.

Sun H, Yang J, Fan B, Ren M, Wang Y, Chen G Int J Mol Sci. 2024; 25(23).

PMID: 39684251 PMC: 11641390. DOI: 10.3390/ijms252312539.

References

Wang H, Wei T, Wang X, Zhang L, Yang M, Chen L . Transcriptome Analyses from Mutant Reveals Important Roles for during Plant Development. Int J Mol Sci. 2018; 19(7). PMC: 6073587. DOI: 10.3390/ijms19072088. View

Ishida M, Hara M, Fukino N, Kakizaki T, Morimitsu Y . Glucosinolate metabolism, functionality and breeding for the improvement of Brassicaceae vegetables. Breed Sci. 2014; 64(1):48-59. PMC: 4031110. DOI: 10.1270/jsbbs.64.48. View

Roxrud I, Lid S, Fletcher J, Schmidt E, Opsahl-Sorteberg H . GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides, regulates flowering and seed development. Plant Cell Physiol. 2007; 48(3):471-83. DOI: 10.1093/pcp/pcm016. View

Wang X, Wang H, Sun R, Wu J, Liu S, Bai Y . The genome of the mesopolyploid crop species Brassica rapa. Nat Genet. 2011; 43(10):1035-9. DOI: 10.1038/ng.919. View

Shi L, Gast R, Gopalraj M, Olszewski N . Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J. 1992; 2(2):153-9. View

Zhang Z, Guo J, Cai X, Li Y, Xi X, Lin R . Improved Reference Genome Annotation of by Pacific Biosciences RNA Sequencing. Front Plant Sci. 2022; 13:841618. PMC: 8968949. DOI: 10.3389/fpls.2022.841618. View

Ahmad B, Yao J, Zhang S, Li X, Zhang X, Yadav V . Genome-Wide Characterization and Expression Profiling of GASA Genes during Different Stages of Seed Development in Grapevine ( L.) Predict Their Involvement in Seed Development. Int J Mol Sci. 2020; 21(3). PMC: 7036793. DOI: 10.3390/ijms21031088. View

Herzog M, Dorne A, Grellet F . GASA, a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene. Plant Mol Biol. 1995; 27(4):743-52. DOI: 10.1007/BF00020227. View

Sun S, Wang H, Yu H, Zhong C, Zhang X, Peng J . GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation. J Exp Bot. 2013; 64(6):1637-47. DOI: 10.1093/jxb/ert021. View

10.

Li X, Shi S, Tao Q, Tao Y, Miao J, Peng X . OsGASR9 positively regulates grain size and yield in rice (Oryza sativa). Plant Sci. 2019; 286:17-27. DOI: 10.1016/j.plantsci.2019.03.008. View

11.

Zhang S, Wang X . Overexpression of GASA5 increases the sensitivity of Arabidopsis to heat stress. J Plant Physiol. 2011; 168(17):2093-101. DOI: 10.1016/j.jplph.2011.06.010. View

12.

Li K, Bai X, Li Y, Cai H, Ji W, Tang L . GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs. J Plant Physiol. 2011; 168(18):2153-60. DOI: 10.1016/j.jplph.2011.07.006. View

13.

Cai C, Wang X, Liu B, Wu J, Liang J, Cui Y . Brassica rapa Genome 2.0: A Reference Upgrade through Sequence Re-assembly and Gene Re-annotation. Mol Plant. 2016; 10(4):649-651. DOI: 10.1016/j.molp.2016.11.008. View

14.

Cai X, Chang L, Zhang T, Chen H, Zhang L, Lin R . Impacts of allopolyploidization and structural variation on intraspecific diversification in Brassica rapa. Genome Biol. 2021; 22(1):166. PMC: 8166115. DOI: 10.1186/s13059-021-02383-2. View

15.

Aubert D, Chevillard M, Dorne A, Arlaud G, Herzog M . Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is up-regulated by gibberellins in meristematic regions. Plant Mol Biol. 1998; 36(6):871-83. DOI: 10.1023/a:1005938624418. View

16.

Rubinovich L, Weiss D . The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta. Plant J. 2010; 64(6):1018-27. DOI: 10.1111/j.1365-313X.2010.04390.x. View

17.

Wang L, Wang Z, Xu Y, Joo S, Kim S, Xue Z . OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice. Plant J. 2008; 57(3):498-510. DOI: 10.1111/j.1365-313X.2008.03707.x. View

18.

Moyano-Canete E, Bellido M, Garcia-Caparros N, Medina-Puche L, Amil-Ruiz F, Gonzalez-Reyes J . FaGAST2, a strawberry ripening-related gene, acts together with FaGAST1 to determine cell size of the fruit receptacle. Plant Cell Physiol. 2012; 54(2):218-36. DOI: 10.1093/pcp/pcs167. View

19.

Huang S, Liu W, Xu J, Liu Z, Li C, Feng H . The SAP function in pistil development was proved by two allelic mutations in Chinese cabbage (Brassica rapa L. ssp. pekinensis). BMC Plant Biol. 2020; 20(1):538. PMC: 7708145. DOI: 10.1186/s12870-020-02741-5. View

20.

Huang D, Wu W, Abrams S, Cutler A . The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J Exp Bot. 2008; 59(11):2991-3007. PMC: 2504347. DOI: 10.1093/jxb/ern155. View