Bone Marrow Mesenchymal Stem Cells Expressing Neat-1, Hotair-1, MiR-21, MiR-644, and MiR-144 Subsided Cyclophosphamide-induced Ovarian Insufficiency by Remodeling the IGF-1-kisspeptin System, Ovarian Apoptosis, and Angiogenesis

Overview

Journal J Ovarian Res

Publisher Biomed Central

Specialties Gynecology & Obstetrics
Reproductive Medicine

Date 2024 Sep 12

PMID 39267091

Authors

Amany I Ahmed

Mohamed F Dowidar

Asmaa F Negm

Hussein Abdellatif

Asma Alanazi

Mohammed Alassiri

Walaa Samy

Dina Mohamed Mekawy

Eman M A Abdelghany

Nesma I El-Naseery

Mohamed A Ibrahem

Emad Ali Albadawi

Wed Salah

Mamdouh Eldesoqui

Emil Tirziu

Iulia Maria Bucur

Ahmed Hamed Arisha

Tarek Khamis

Affiliations

Soon will be listed here.

Abstract

Ovarian insufficiency is one of the common reproductive disorders affecting women with limited therapeutic aids. Mesenchymal stem cells have been investigated in such disorders before yet, the exact mechanism of MSCs in ovarian regeneration regarding their epigenetic regulation remains elusive. The current study is to investigate the role of the bone marrow-derived mesenchymal stem cells (BM-MSCs) lncRNA (Neat-1 and Hotair1) and miRNA (mir-21-5p, mir-144-5p, and mir-664-5p) in mitigating ovarian granulosa cell apoptosis as well as searching BM-MSCs in altering the expression of ovarian and hypothalamic IGF-1 - kisspeptin system in connection to HPG axis in a cyclophosphamide-induced ovarian failure rat model. Sixty mature female Sprague Dawley rats were divided into 3 equal groups; control group, premature ovarian insufficiency (POI) group, and POI + BM-MSCs. POI female rat model was established with cyclophosphamide. The result revealed that BM-MSCs and their conditioned media displayed a significant expression level of Neat-1, Hotair-1, mir-21-5p, mir-144-5p, and mir-664-5p. Moreover, BM-MSCs transplantation in POI rats improves; the ovarian and hypothalamic IGF-1 - kisspeptin, HPG axis, ovarian granulosa cell apoptosis, steroidogenesis, angiogenesis, energy balance, and oxidative stress. BM-MSCs expressed higher levels of antiapoptotic lncRNAs and microRNAs that mitigate ovarian insufficiency.

Citing Articles

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Cui X, Li H, Zhu X, Huang X, Xue T, Wang S Apoptosis. 2025; .

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The Functions and Implications of MicroRNAs in Premature Ovarian Insufficiency.

Gao H, Cao X, Hua H, Zhu H Mol Genet Genomic Med. 2025; 13(2):e70074.

PMID: 39959946 PMC: 11831191. DOI: 10.1002/mgg3.70074.

CircTEC Inhibits the Follicular Atresia in Buffalo () via Targeting miR-144-5p/FZD3 Signaling Axis.

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Correction: Bone marrow mesenchymal stem cells expressing Neat-1, Hotair-1, miR-21, miR-644, and miR-144 subsided cyclophosphamide-induced ovarian insufficiency by remodeling the IGF-1-kisspeptin system, ovarian apoptosis, and angiogenesis.

Ahmed A, Dowidar M, Negm A, Abdellatif H, Alanazi A, Alassiri M J Ovarian Res. 2024; 17(1):239.

PMID: 39614263 PMC: 11606127. DOI: 10.1186/s13048-024-01564-4.

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