Oncostatin M Receptor-dependent Signaling Assessed by RNA Sequencing in Mouse Hematopoietic Stem Cells

Overview

Journal Sci Data

Specialty Science

Date 2024 Sep 12

PMID 39266541

Authors

Logan S Schwartz

Ruth L Saxl

Tim Stearns

Maria Telpoukhovskaia

Jennifer J Trowbridge

Affiliations

Soon will be listed here.

Abstract

Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmr mouse model B6;129-Osmr/J. We crossed Osmr mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ -dependent and -independent OSM signaling as well as the transcriptional effects of an IL-6 family cytokine on mouse HSCs to further define its anti-inflammatory versus pro-inflammatory properties.

Citing Articles

Oncostatin M receptor-dependent signaling assessed by RNA sequencing in mouse hematopoietic stem cells.

Schwartz L, Saxl R, Stearns T, Telpoukhovskaia M, Trowbridge J Sci Data. 2024; 11(1):996.

PMID: 39266541 PMC: 11392925. DOI: 10.1038/s41597-024-03839-3.

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