Bioassay-Guided Fractionation of Acetone and Methanol Extracts of Galls with Antimalarial Properties

Overview

Journal Trop Life Sci Res

Date 2024 Sep 5

PMID 39234468

Authors

Nurul Hammizah Hamidon

Anjana Chamilka Thuduhenage Dona

Nik Nor Imam Nik Mat Zin

Nurul Izza Nordin

Shaida Fariza Sulaiman

Nurhidanatasha Abu-Bakar

Affiliations

Soon will be listed here.

Abstract

The antimalarial properties of crude extracts from galls were investigated through bioassay-guided fractionation. Acetone (QIA) and methanol (QIM) crude extracts have been reported to have promising antimalarial activity against (3D7 strain). These extracts were subjected to fractionation using automated preparative high-performance liquid chromatography (prep-HPLC) to identify the most active fractions. Nine fractions were isolated from each extract, of which the fractions QIA11 and QIM16 showed antimalarial activity, with IC values of 17.65 ± 1.82 μg/mL and 24.21 ± 1.88 μg/mL, respectively. In comparison, the standard antimalarial drug artemisinin has an IC value of 0.004 ± 0.001 μg/mL). Through high-resolution liquid chromatography coupled with mass spectrometry (HR-LCMS) analysis of the fractions, four known compounds were successfully identified: gallic acid, ellagic acid, 1,3,6-tris-o-(3,4,5-trihydroxybenzoyl)-beta-d-glucose and 1-O,6-O-digalloyl-beta-D-glucose.

References

Ekasari W, Fatmawati D, Khoiriah S, Baqiuddin W, Nisa H, Maharupini A . Antimalarial Activity of Extract and Fractions of (L.) Merr. Scientifica (Cairo). 2022; 2022:3552491. PMC: 9477630. DOI: 10.1155/2022/3552491. View

Che C, Zhang H . Plant Natural Products for Human Health. Int J Mol Sci. 2019; 20(4). PMC: 6412953. DOI: 10.3390/ijms20040830. View

Newman D, Cragg G . Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. J Nat Prod. 2020; 83(3):770-803. DOI: 10.1021/acs.jnatprod.9b01285. View

Kumar N, Goel N . Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep (Amst). 2019; 24:e00370. PMC: 6734135. DOI: 10.1016/j.btre.2019.e00370. View

Arya A, Kojom Foko L, Chaudhry S, Sharma A, Singh V . Artemisinin-based combination therapy (ACT) and drug resistance molecular markers: A systematic review of clinical studies from two malaria endemic regions - India and sub-Saharan Africa. Int J Parasitol Drugs Drug Resist. 2021; 15:43-56. PMC: 7887327. DOI: 10.1016/j.ijpddr.2020.11.006. View

Shrestha S, Kaushik V, Eshwarappa R, Subaramaihha S, Ramanna L, Lakkappa D . Pharmacognostic studies of insect gall of Quercus infectoria Olivier (Fagaceae). Asian Pac J Trop Biomed. 2013; 4(1):35-9. PMC: 3819493. DOI: 10.1016/S2221-1691(14)60205-7. View

Uzor P, Onyishi C, Omaliko A, Nworgu S, Ugwu O, Nwodo N . Study of the Antimalarial Activity of the Leaf Extracts and Fractions of and and Their HPLC Analysis. Evid Based Complement Alternat Med. 2021; 2021:5218294. PMC: 8313334. DOI: 10.1155/2021/5218294. View

Yuan H, Ma Q, Ye L, Piao G . The Traditional Medicine and Modern Medicine from Natural Products. Molecules. 2016; 21(5). PMC: 6273146. DOI: 10.3390/molecules21050559. View

Zin N, Mohamad M, Roslan K, Abdul Wafi S, Abdul Moin N, Alias A . In Vitro Antimalarial and Toxicological Activities of (Olivier) Gall Extracts. Malays J Med Sci. 2020; 27(4):36-50. PMC: 7444841. DOI: 10.21315/mjms2020.27.4.4. View

10.

Nigussie G, Wale M . Medicinal plants used in traditional treatment of malaria in Ethiopia: a review of ethnomedicine, anti-malarial and toxicity studies. Malar J. 2022; 21(1):262. PMC: 9463824. DOI: 10.1186/s12936-022-04264-w. View

11.

Shah G, Abbasi A, Khan N, Guo X, Khan M, Hussain M . Traditional uses of medicinal plants against malarial disease by the tribal communities of Lesser Himalayas-Pakistan. J Ethnopharmacol. 2014; 155(1):450-62. DOI: 10.1016/j.jep.2014.05.047. View

12.

Ouji M, Augereau J, Paloque L, Benoit-Vical F . Plasmodium falciparum resistance to artemisinin-based combination therapies: A sword of Damocles in the path toward malaria elimination. Parasite. 2018; 25:24. PMC: 5909375. DOI: 10.1051/parasite/2018021. View

13.

Basri D, Tan L, Shafiei Z, Mohamad Zin N . In Vitro Antibacterial Activity of Galls of Quercus infectoria Olivier against Oral Pathogens. Evid Based Complement Alternat Med. 2011; 2012:632796. PMC: 3235900. DOI: 10.1155/2012/632796. View

14.

Ma S, Qin H, Jiang M, Wang J, Wang W, Guo G . Identification and Comparison of Tannins in Gall of Rhus chinensis Mill. and Gall of Quercus infectoria Oliv. by High-Performance Liquid Chromatography-Electrospray Mass Spectrometry. J Chromatogr Sci. 2020; 58(5):403-410. DOI: 10.1093/chromsci/bmz096. View

15.

Hamid H, Kaur G, Abdullah S, Ali M, Athar M, Alam M . Two New Compounds from the Galls of Quercus infectoria. with Nitric Oxide and Superoxide Inhibiting Ability. Pharm Biol. 2017; 43(4):317-323. DOI: 10.1080/13880200590951711. View

16.

Jonville M, Kodja H, Humeau L, FOURNEL J, De Mol P, Cao M . Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity. J Ethnopharmacol. 2008; 120(3):382-6. DOI: 10.1016/j.jep.2008.09.005. View

17.

Ferrer Amate C, Unterluggauer H, Fischer R, Fernandez-Alba A, Masselter S . Development and validation of a LC-MS/MS method for the simultaneous determination of aflatoxins, dyes and pesticides in spices. Anal Bioanal Chem. 2010; 397(1):93-107. DOI: 10.1007/s00216-010-3526-x. View

18.

Wu Z, Gao W, Phelps M, Wu D, Miller D, Dalton J . Favorable effects of weak acids on negative-ion electrospray ionization mass spectrometry. Anal Chem. 2004; 76(3):839-47. PMC: 2104782. DOI: 10.1021/ac0351670. View

19.

Kamarudin N, Nik Salleh N, Tan S . Gallotannin-Enriched Fraction from Galls as an Antioxidant and Inhibitory Agent against Human Glioblastoma Multiforme. Plants (Basel). 2021; 10(12). PMC: 8708140. DOI: 10.3390/plants10122581. View

20.

Tayel A, El-Sedfy M, Ibrahim A, Moussa S . Application of Quercus infectoria extract as a natural antimicrobial agent for chicken egg decontamination. Rev Argent Microbiol. 2018; 50(4):391-397. DOI: 10.1016/j.ram.2017.12.003. View