A Highly Conserved A-to-I RNA Editing Event Within the Glutamate-gated Chloride Channel GluClα is Necessary for Olfactory-based Behaviors in

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2024 Sep 4

PMID 39231215

Authors

Hila Zak

Eyal Rozenfeld

Mali Levi

Patricia Deng

David Gorelick

Hadar Pozeilov

Shai Israel

Yoav Paas

Jin Billy Li

Moshe Parnas

Galit Shohat-Ophir

Affiliations

Soon will be listed here.

Abstract

A-to-I RNA editing is a cellular mechanism that generates transcriptomic and proteomic diversity, which is essential for neuronal and immune functions. It involves the conversion of specific adenosines in RNA molecules to inosines, which are recognized as guanosines by cellular machinery. Despite the vast number of editing sites observed across the animal kingdom, pinpointing critical sites and understanding their in vivo functions remains challenging. Here, we study the function of an evolutionary conserved editing site in , located in glutamate-gated chloride channel (α). Our findings reveal that flies lacking editing at this site exhibit reduced olfactory responses to odors and impaired pheromone-dependent social interactions. Moreover, we demonstrate that editing of this site is crucial for the proper processing of olfactory information in projection neurons. Our results highlight the value of using evolutionary conservation as a criterion for identifying editing events with potential functional significance and paves the way for elucidating the intricate link between RNA modification, neuronal physiology, and behavior.

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