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Phthalate Metabolism in Pseudomonas Fluorescens PHK: Purification and Properties of 4,5-dihydroxyphthalate Decarboxylase

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1985 Feb 1
PMID 3920963
Citations 14
Authors
B G Pujar
D W Ribbons
Affiliations
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Abstract

Pseudomonas fluorescens PHK uses 4,5-dihydroxyphthalate as the sole carbon source for o-phthalate catabolism. This intermediate is the substrate for a decarboxylase of the pathway yielding protocatechuate. The decarboxylase was purified to homogeneity by an affinity chromatography procedure in which the reaction product, protocatechuate, was used as a ligand. We describe some properties of the enzyme, including its apparent molecular weight of 420,000 as determined by gel filtration and of 66,000 after sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, consistent with a hexameric functional protein. The apparent Km for the substrate 4,5-dihydroxyphthalate was 10.4 microM. The characteristics of this enzyme are compared with those described for the isofunctional enzyme from P. testosteroni.

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