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DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products

Overview
Journal Foods
Specialty Biotechnology
Date 2024 Aug 29
PMID 39200507
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Abstract

Snow Lotus Seed (SLS), esteemed for its nutritional and market value, faces challenges of authentication due to the absence of appropriate testing standards and methods. This results in frequent adulteration of SLS sourced from () with other plant seeds endosperm. Traditional chloroplast DNA barcoding methods are inadequate for species identification due to the absence of chloroplasts in seeds endosperm. In this study, the homology of 11 ITS genes among 6 common species was analyzed. Universal primers suitable for these species were designed and screened. A DNA barcoding method for distinguishing SLS species was developed using Sanger sequencing technology, leveraging existing GenBank and Barcode of Life Data System (BOLD) databases. Optimized sample pretreatment facilitated effective DNA extraction from phytopolysaccharide-rich SLS. Through testing of commercial SLS products, the species origin has been successfully identified. Additionally, a novel instance of food fraud was uncovered, where the endosperm was used to counterfeit SLS for the first time. The study established that the developed DNA barcoding method is effective for authenticating SLS species. It is of great significance for combating food fraud related to SLS, ensuring food safety, and promoting the healthy development of the SLS industry.

References
1.
Qian Z, Munywoki J, Wang Q, Malombe I, Li Z, Chen J . Molecular Identification of African Species (Water Lily) Based on ITS, and . Plants (Basel). 2022; 11(18). PMC: 9503883. DOI: 10.3390/plants11182431. View

2.
Yang X, Yu X, Zhang X, Guo H, Xing Z, Xu L . Development of Mini-Barcode Based on Chloroplast Genome and Its Application in Metabarcoding Molecular Identification of Chinese Medicinal Material Radix (Chishao). Front Plant Sci. 2022; 13:819822. PMC: 9009180. DOI: 10.3389/fpls.2022.819822. View

3.
Chen S, Yao H, Han J, Liu C, Song J, Shi L . Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One. 2010; 5(1):e8613. PMC: 2799520. DOI: 10.1371/journal.pone.0008613. View

4.
Fierro O, Siano F, Bianco M, Vasca E, Picariello G . Comprehensive molecular level characterization of protein- and polyphenol-rich tara (Caesalpinia spinosa) seed germ flour suggests novel hypothesis about possible accidental hazards. Food Res Int. 2024; 181:114119. DOI: 10.1016/j.foodres.2024.114119. View

5.
Healey A, Furtado A, Cooper T, Henry R . Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species. Plant Methods. 2014; 10:21. PMC: 4105509. DOI: 10.1186/1746-4811-10-21. View