Dissociated Production of Interleukin-2 and Immune (gamma) Interferon by Phytohaemagglutinin Stimulated Lymphocytes in Healthy Infants

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1985 Feb 1

PMID 3919982

Citations 20

Authors

T Miyawaki

H Seki

K Taga

H Sato

N Taniguchi

Affiliations

Soon will be listed here.

Abstract

Cord blood lymphocytes were stimulated with phytohaemagglutinin (PHA) to produce interleukin-2 (IL-2) and immune interferon (IFN-gamma). On PHA stimulation, cord blood lymphocytes produced efficiently IL-2 as much as adult ones. Antiviral activity generated on PHA stimulation was shown to consist mainly of IFN-gamma as assessed by the sensitivity to pH 2.0 treatment and neutralization with anti-human IFN-gamma antibody. In contrast to IL-2 production, cord blood lymphocytes released extremely low levels of IFN-gamma following PHA stimulation. The producing ability of IFN-gamma by lymphocytes on PHA stimulation gradually increased with child growth, but was significantly low at 1-2 years of age as compared with adult controls. Around 3 years of age or later, the producing ability of IFN-gamma by lymphocytes on PHA stimulation attained levels comparable to those of adult cells. These results suggested that IL-2 producing ability of lymphocytes appeared to be at a mature stage at birth, whereas lymphocytes in the early human life might be relatively deficient in their ability to produce IFN-gamma.

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