Impact of Carbon Dioxide Concentrations on Laboratory Sensitivity of Species Isolated from Dairy Cows

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2024 Aug 20

PMID 39162555

Authors

Marit M Biesheuvel

Kristen Kalbfleisch

Jeroen De Buck

Erik van Engelen

Gerdien van Schaik

Paolo Moroni

Gloria Gioia

Diego Nobrega

Gennerick J Samera

Paul J Adams

Jermaine R Walcott

Herman W Barkema

Affiliations

Soon will be listed here.

Abstract

Importance: Current spp. culture protocols lack empirical derivation concerning carbon dioxide (CO) supplementation and are primarily based on the initial cultivation of . This study indicates that the suitable range for CO supplementation is broader than what is currently recommended by the National Mastitis Council for culturing within the specified 7-10 days. No differences in bacterial growth detection rates were observed among ambient air, 5% CO, or 10% CO supplementation during the 7- and 10-day incubation intervals. These new insights provide evidence supporting the possibility of culturing spp. under ambient air conditions in a laboratory setting.

References

Tao Z, Yuan H, Liu M, Liu Q, Zhang S, Liu H . Yeast Extract: Characteristics, Production, Applications and Future Perspectives. J Microbiol Biotechnol. 2022; 33(2):151-166. PMC: 9998214. DOI: 10.4014/jmb.2207.07057. View

Jolley K, Maiden M . BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics. 2010; 11:595. PMC: 3004885. DOI: 10.1186/1471-2105-11-595. View

Gonzalez R, Wilson D . Mycoplasmal mastitis in dairy herds. Vet Clin North Am Food Anim Pract. 2003; 19(1):199-221. DOI: 10.1016/s0749-0720(02)00076-2. View

Johnson M, Hill S, Piddock L . Effect of carbon dioxide on testing of susceptibilities of respiratory tract pathogens to macrolide and azalide antimicrobial agents. Antimicrob Agents Chemother. 1999; 43(8):1862-5. PMC: 89381. DOI: 10.1128/AAC.43.8.1862. View

Heuer H, Krsek M, Baker P, Smalla K, Wellington E . Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl Environ Microbiol. 1997; 63(8):3233-41. PMC: 168621. DOI: 10.1128/aem.63.8.3233-3241.1997. View

Cummins E, Selfridge A, Sporn P, Sznajder J, Taylor C . Carbon dioxide-sensing in organisms and its implications for human disease. Cell Mol Life Sci. 2013; 71(5):831-45. PMC: 3945669. DOI: 10.1007/s00018-013-1470-6. View

Murai K, Lehenbauer T, Champagne J, Glenn K, Aly S . Cost-effectiveness of diagnostic strategies using quantitative real-time PCR and bacterial culture to identify contagious mastitis cases in large dairy herds. Prev Vet Med. 2014; 113(4):522-35. DOI: 10.1016/j.prevetmed.2014.01.001. View

Cai H, Bell-Rogers P, Parker L, Prescott J . Development of a real-time PCR for detection of Mycoplasma bovis in bovine milk and lung samples. J Vet Diagn Invest. 2006; 17(6):537-45. DOI: 10.1177/104063870501700603. View

Aebi M, Bodmer M, Frey J, Pilo P . Herd-specific strains of Mycoplasma bovis in outbreaks of mycoplasmal mastitis and pneumonia. Vet Microbiol. 2012; 157(3-4):363-8. DOI: 10.1016/j.vetmic.2012.01.006. View

10.

SHEPARD M, LUNCEFORD C . EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965; 89:265-70. PMC: 305502. DOI: 10.1128/jb.89.2.265-270.1965. View

11.

Davami F, Baldi L, Rajendra Y, Wurm F . Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells. Int J Mol Cell Med. 2014; 3(3):146-56. PMC: 4170488. View

12.

Caswell J, Archambault M . Mycoplasma bovis pneumonia in cattle. Anim Health Res Rev. 2008; 8(2):161-86. DOI: 10.1017/S1466252307001351. View

13.

HALE H, Helmboldt C, PLASTRIDGE W, STULA E . Bovine mastitis caused by a Mycoplasma species. Cornell Vet. 1962; 52:582-91. View

14.

Nicholas R, Ayling R . Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci. 2003; 74(2):105-12. DOI: 10.1016/s0034-5288(02)00155-8. View

15.

Nicholas R, Baker S . Recovery of mycoplasmas from animals. Methods Mol Biol. 1998; 104:37-43. DOI: 10.1385/0-89603-525-5:37. View

16.

Erno H, PLASTRIDGE W, Tourtellotte M . Mycoplasma: nutritional studies of laboratory strains. Acta Vet Scand. 1967; 8(2):111-22. PMC: 8753744. View

17.

Pfutzner H, Sachse K . Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Rev Sci Tech. 1996; 15(4):1477-94. DOI: 10.20506/rst.15.4.987. View

18.

Arcangioli M, Chazel M, Sellal E, Botrel M, Bezille P, Poumarat F . Prevalence of Mycoplasma bovis udder infection in dairy cattle: preliminary field investigation in southeast France. N Z Vet J. 2011; 59(2):75-8. DOI: 10.1080/00480169.2011.552856. View

19.

Biesheuvel M, Ward C, Penterman P, van Engelen E, van Schaik G, Deardon R . Within-herd transmission of Mycoplasma bovis infections after initial detection in dairy cows. J Dairy Sci. 2023; 107(1):516-529. DOI: 10.3168/jds.2023-23407. View

20.

Vahanikkila N, Pohjanvirta T, Haapala V, Simojoki H, Soveri T, Browning G . Characterisation of the course of Mycoplasma bovis infection in naturally infected dairy herds. Vet Microbiol. 2019; 231:107-115. DOI: 10.1016/j.vetmic.2019.03.007. View