Satellite Cell-derived TRIM28 is Pivotal for Mechanical Load- and Injury-induced Myogenesis

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2024 Aug 14

PMID 39143258

Authors

Kuan-Hung Lin

Jamie E Hibbert

Corey Gk Flynn

Jake L Lemens

Melissa M Torbey

Nathaniel D Steinert

Philip M Flejsierowicz

Kiley M Melka

Garrison T Lindley

Marcos Lares

Vijayasaradhi Setaluri

Amy J Wagers

Troy A Hornberger

Affiliations

Soon will be listed here.

Abstract

Satellite cells are skeletal muscle stem cells that contribute to postnatal muscle growth, and they endow skeletal muscle with the ability to regenerate after a severe injury. Here we discover that this myogenic potential of satellite cells requires a protein called tripartite motif-containing 28 (TRIM28). Interestingly, different from the role reported in a previous study based on C2C12 myoblasts, multiple lines of both in vitro and in vivo evidence reveal that the myogenic function of TRIM28 is not dependent on changes in the phosphorylation of its serine 473 residue. Moreover, the functions of TRIM28 are not mediated through the regulation of satellite cell proliferation or differentiation. Instead, our findings indicate that TRIM28 regulates the ability of satellite cells to progress through the process of fusion. Specifically, we discover that TRIM28 controls the expression of a fusogenic protein called myomixer and concomitant fusion pore formation. Collectively, the outcomes of this study expose the framework of a novel regulatory pathway that is essential for myogenesis.

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