Altered Levels of IFN-γ, IL-4, and IL-5 Depend on the Rs4986790 Genotype in COPD Smokers but Not Those Exposed to Biomass-burning Smoke

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2024 Aug 14

PMID 39139567

Authors

Karla Jazmin Gutierrez-Romero

Ramces Falfan-Valencia

Alejandra Ramirez-Venegas

Rafael De Jesus Hernandez-Zenteno

Fernando Flores-Trujillo

Raul Sansores-Martinez

Espiridion Ramos-Martinez

Gloria Perez-Rubio

Affiliations

Soon will be listed here.

Abstract

Introduction: Chronic obstructive pulmonary disease (COPD) is associated with tobacco smoking and biomass-burning smoke exposure. Toll-like receptor 4 () single-nucleotide polymorphisms (SNPs) may contribute to its pathogenesis. The study aimed to assess the association of rs4986790 and rs4986791 in the gene in a Mexican mestizo population with COPD secondary to tobacco smoking (COPD-TS) and biomass-burning smoke (COPD-BBS) and to evaluate whether the genotypes of risk affect cytokine serum levels.

Materials And Methods: We enrolled 2,092 participants and divided them into two comparisons according to their environmental exposure. SNPs were genotyped using TaqMan probes. Serum cytokine levels (IL-4, IL-5, IL-6, IL-10, and INF-γ) were quantified by ELISA.

Results: The rs4986790 AA genotype in COPD-TS was associated with a higher COPD risk (OR = 3.53). Haplotype analysis confirmed this association, identifying a block containing the rs4986790 allele (A-C, OR = 3.11). COPD-TS exhibited elevated IL-6, IL-4, and IL-5 levels compared with smokers without COPD (SWOC), whereas COPD-BBS displayed higher IFN-γ, IL-6, and IL-10 levels. The AA carriers in the COPD-TS group had elevated IL-4, IL-5, and IFN-γ compared with carriers of AG or GG.

Conclusion: The rs4986790 common allele and the A-C haplotype (rs4986790-rs4986791) were associated with a higher COPD risk in smokers; COPD patients carrying the AA genotype showed increased pro-inflammatory cytokines.

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