Combined Treatment with Vitamin C, Hydrocortisone and Thiamine Does Not Attenuate Morbidity and Mortality of Septic Sheep

Overview

Journal Lab Anim Res

Date 2024 Aug 12

PMID 39135077

Authors

Tuvshintugs Baljinnyam

Satoshi Fukuda

Yosuke Niimi

Donald Prough

Perenlei Enkhbaatar

Affiliations

Soon will be listed here.

Abstract

Background: Sepsis is associated with a highest mortality rate in the ICU. Present study tests the efficacy of combined therapy with vitamin C, hydrocortisone and thiamine (combined therapy) in the ovine model of sepsis induced by Pseudomonas aeruginosa. In this study, sepsis was induced in sheep by instillation of Pseudomonas aeruginosa (1 × 10 CFU) into the lungs via bronchoscope, under anesthesia. Nine hours after injury, intravenous infusion of vitamin C (0.75 g every 6 h), hydrocortisone (25 mg every 6 h), and thiamine (100 mg every 12 h) or saline was given to the treatment and control groups. Cardiopulmonary variables were recorded.

Results: The survival rate was 16.7% in control and 33.3% in treatment groups. In the control group, mean arterial pressure dropped from 93.6 ± 8.6 to 75.5 ± 9.7 mmHg by 9 h, which was not affected by the combined therapy. Pulmonary dysfunction was not attenuated by the combined therapy either. The combined therapy had no effect on increased extravascular lung water content and fluid effusion into thoracic cavity. The bacterial number in the bronchoalveolar lavage fluid was significantly increased in the treatment group than the control group. The blood bacterial number remained comparable between groups.

Conclusions: Combined vitamin C, hydrocortisone, and thiamine did not attenuate severity of ovine sepsis.

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