Analyses of Neurite and Spine Formation by In Utero Electroporation in Mice
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Abstract
Dendrite morphology and dendritic spines are key features of the neuronal networks in the brain. Abnormalities in these features have been observed in patients with psychiatric disorders and mouse models of these diseases. In utero electroporation is an easy and efficient gene transfer system for developing mouse embryos in the uterus. By combining with the Cre-loxP system, the morphology of individual neurons can be clearly and sparsely visualized. Here, we describe how this labeling system can be applied to visualize and evaluate the dendrites and dendritic spines of cortical neurons.
References
1.
Martinez-Cerdeno V
. Dendrite and spine modifications in autism and related neurodevelopmental disorders in patients and animal models. Dev Neurobiol. 2016; 77(4):393-404.
PMC: 5219951.
DOI: 10.1002/dneu.22417.
View
2.
Tabata H, Nakajima K
. Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex. Neuroscience. 2001; 103(4):865-72.
DOI: 10.1016/s0306-4522(01)00016-1.
View
3.
Grove E
. Neocortex patterning by the secreted signaling molecule FGF8. Science. 2001; 294(5544):1071-4.
DOI: 10.1126/science.1064252.
View
4.
Saito T, Nakatsuji N
. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol. 2002; 240(1):237-46.
DOI: 10.1006/dbio.2001.0439.
View
5.
Ito H, Morishita R, Iwamoto I, Nagata K
. Establishment of an in vivo electroporation method into postnatal newborn neurons in the dentate gyrus. Hippocampus. 2014; 24(12):1449-57.
DOI: 10.1002/hipo.22325.
View