Genome Wide Conditional Mouse Knockout Resources

Overview

Journal Drug Discov Today Dis Models

Publisher Elsevier

Date 2024 Aug 12

PMID 39132094

Authors

C Kaloff

K Anastassiadis

A Ayadi

R Baldock

J Beig

M-C Birling

A Bradley

S Brown

A Burger

W Bushell

F Chiani

F S Collins

B Doe

J T Eppig

R H Finnel

C Fletcher

P Flicek

M Fray

R H Friedel

A Gambadoro

H Gates

J Hansen

Y Herault

G G Hicks

A Horlein

M Hrabe de Angelis

V Iyer

P J de Jong

G Koscielny

R Kuhn

P Liu

K C Lloyd

R G Lopez

S Marschall

S Martinez

C McKerlie

T Meehan

H von Melchner

M Moore

S A Murray

A Nagy

Lmj Nutter

G Pavlovic

A Pombero

H Prosser

R Ramirez-Solis

M Ringwald

B Rosen

N Rosenthal

J Rossant

P Ruiz Noppinger

E Ryder

W C Skarnes

J Schick

F Schnutgen

P Schofield

C Seisenberger

M Selloum

D Smedley

E M Simpson

A F Stewart

L Teboul

G P Tocchini Valentini

D Valenzuela

A West

W Wurst

Affiliations

Soon will be listed here.

Abstract

The International Knockout Mouse Consortium (IKMC) developed high throughput gene trapping and gene targeting pipelines that produced mostly conditional mutations of more than 18,500 genes in C57BL/6N mouse embryonic stem (ES) cells which have been archived and are freely available to the research community as a frozen resource. From this unprecedented resource more than 6,000 mutant mouse strains have been produced by the IKMC and mostly the International Mouse Phenotyping Consortium (IMPC). In addition, a cre-driver resource was established including 250 inducible cre-driver mouse strains in a C57BL/6 background. Complementing the cre-driver resource, a collection of comprising 27 cre-driver rAAVs has also been produced. The resources can be easily accessed at the IKMC/IMPC web portal (www.mousephenotype.org). The IKMC/IMPC resource is a standardized reference library of mouse models with defined genetic backgrounds that enables the analysis of gene-disease associations in mice of different genetic makeup and should therefore have a major impact on biomedical research.

Citing Articles

Advances in mouse genetics for the study of human disease.

Brown S Hum Mol Genet. 2021; 30(R2):R274-R284.

PMID: 34089057 PMC: 8490014. DOI: 10.1093/hmg/ddab153.

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