MiR-203-3p Promotes Senescence of Mouse Bone Marrow Mesenchymal Stem Cells Via Downregulation of Pbk

Overview

Journal Aging Cell

Specialties Cell Biology
Geriatrics

Date 2024 Aug 9

PMID 39123275

Authors

Qiaojuan Mei

Kexin Li

Tianyu Tang

Siying Cai

Yu Liu

Xiaofei Wang

Yinzhao Jia

Ling Zhang

Huaibiao Li

Hui Song

Jun Zhai

Wenpei Xiang

Affiliations

Soon will be listed here.

Abstract

The senescence of bone marrow mesenchymal stem cells (BMSCs) contributes to the development of degenerative skeletal conditions. To date, the molecular mechanism resulting in BMSC senescence has not been fully understood. In this study, we identified a small non-coding RNA, miR-203-3p, the expression of which was elevated in BMSCs from aged mice. On the other hand, overexpression of miR-203-3p in BMSCs from young mice reduced cell growth and enhanced their senescence. Mechanistically, PDZ-linked kinase (PBK) is predicted to be the target of miR-203-3p. The binding of miR-203-3p to Pbk mRNA could decrease its expression, which in turn inhibited the ubiquitination-mediated degradation of p53. Furthermore, the intravitreal injection of miR-203-3p-inhibitor into the bone marrow cavity of aged mice attenuated BMSC senescence and osteoporosis in aged mice. Collectively, these findings suggest that targeting miR-203-3p to delay BMSC senescence could be a potential therapeutic strategy to alleviate age-related osteoporosis.

Citing Articles

miR-203-3p promotes senescence of mouse bone marrow mesenchymal stem cells via downregulation of Pbk.

Mei Q, Li K, Tang T, Cai S, Liu Y, Wang X Aging Cell. 2024; 23(11):e14293.

PMID: 39123275 PMC: 11561657. DOI: 10.1111/acel.14293.

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