CLG Promotes MTOR/ULK1 Pathway-mediated Autophagy to Inhibit OS Development by Inhibiting TRAF6-mediated FLT3 Ubiquitination

Overview

Journal Cancer Sci

Specialty Oncology

Date 2024 Aug 9

PMID 39118482

Authors

Xiongjie Huang

Yanran Huang

Bin Peng

Junfang Wang

Huiyu Tang

Yanming Chen

Affiliations

Soon will be listed here.

Abstract

Corilagin (CLG) has antitumor activities in certain human malignant cancers. Herein, the effects and mechanisms of CLG on osteosarcoma (OS) were investigated. OS cell viability and proliferation were detected by MTT and colony formation assay. Cell cycle and apoptosis were examined using flow cytometry. The interaction between TRAF6 and FLT3 was investigated using a co-immunoprecipitation assay. Results demonstrated that CLG treatment inhibited OS cell viability and proliferation but promoted OS cell autophagy and apoptosis in a concentration-dependent manner. Mechanically, CLG inhibited TRAF6-mediated FLT3 ubiquitination degradation. TRAF6 overexpression abolished the effects of CLG on OS cell proliferation, autophagy, and apoptosis. Finally, CLG administration inhibited OS tumor growth in mice by inducing autophagy-dependent apoptosis. Taken together, CLG inhibited OS progression by facilitating mTOR/ULK1 pathway-mediated autophagy through inhibiting TRAF6-mediated FLT3 ubiquitination, which indicated that CLG was a promising candidate for the treatment of OS.

Citing Articles

Study on the Effects and Mechanism of Corilagin on A2780 Cell Apoptosis.

Xu Z, Jiang Y, Shan T, Hu L, Wu M, Ji H Curr Issues Mol Biol. 2025; 47(2).

PMID: 39996826 PMC: 11854497. DOI: 10.3390/cimb47020105.

CLG promotes mTOR/ULK1 pathway-mediated autophagy to inhibit OS development by inhibiting TRAF6-mediated FLT3 ubiquitination.

Huang X, Huang Y, Peng B, Wang J, Tang H, Chen Y Cancer Sci. 2024; 115(10):3466-3480.

PMID: 39118482 PMC: 11447880. DOI: 10.1111/cas.16274.

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