The Frequency and Function of Nucleoprotein-specific CD8 T Cells Are Critical for Heterosubtypic Immunity Against Influenza Virus Infection

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2024 Jul 31

PMID 39082839

Authors

Samuel Amoah

Weiping Cao

Ekramy E Sayedahmed

Yuanyuan Wang

Amrita Kumar

Margarita Mishina

Devon J Eddins

Wen-Chien Wang

Mark Burroughs

Mili Sheth

Justin Lee

Wun-Ju Shieh

Sean D Ray

Caitlin D Bohannon

Priya Ranjan

Suresh D Sharma

Jessica Hoehner

Robert A Arthur

Shivaprakash Gangappa

Nobuko Wakamatsu

H Richard Johnston

Jan Pohl

Suresh K Mittal

Suryaprakash Sambhara

Affiliations

Soon will be listed here.

Abstract

Importance: Cytotoxic T lymphocytes (CTLs) are an important component of the adaptive immune system that clears virus-infected cells or tumor cells. Hence, developing next-generation vaccines that induce or recall CTL responses against cancer and infectious diseases is crucial. However, it is not clear if the frequency, function, or both are essential in conferring protection, as in the case of influenza. In this study, we demonstrate that both CTL frequency and function are crucial for providing heterosubtypic immunity to influenza by utilizing an Ad-viral vector expressing a CD8 epitope only to rule out the role of antibodies, single-cell RNA-seq analysis, as well as adoptive transfer experiments. Our findings have implications for developing T cell vaccines against infectious diseases and cancer.

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