Promising Approach for Targeting ROBO1 with CAR NK Cells to Combat Ovarian Cancer Primary Tumor Cells and Organoids

Overview

Journal Future Sci OA

Specialty Biotechnology

Date 2024 Jul 29

PMID 39069888

Authors

Yan Zhu

Luanhong Wang

Biyu Jiang

Yini Wang

Qing Wu

Sihua Hong

Xiaojing Wang

Yuancheng Li

Tian Guan

Haoyu Zeng

Congzhu Li

Affiliations

Soon will be listed here.

Abstract

This study aimed to explore using peripheral blood mononuclear cell (PBMC)-derived chimeric antigen receptor (CAR) NK cells targeting ROBO1 as a personalized medicine approach for ovarian cancer. A two-step strategy generated ROBO1-targeted CAR NK cells from PBMCs of ovarian cancer patients. Efficacy was evaluated using xCELLigence RTCA, CCK-8 and Live/Dead fluorescence assays. ROBO1-NK cells exhibited higher efficiency in eradicating primary ovarian cancer cells and lysing ovarian tumor organoids compared with primary NK cells without ROBO1-CAR modification. These findings highlight the potential of developing ROBO1-targeted CAR-NK cells from patients' PBMCs as a personalized treatment option for ovarian cancer.

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